中国白菜型油菜种质资源的遗传多样性研究

利用RAPD标记和UPGMA聚类分析, 对我国23个省市的172份白菜型油菜资源进行了遗传多样性分析. 43个随机引物扩增出248条多态性带. 聚类分析表明, 我国白菜型油菜分为15个类群, 其中, 6个类群为北方小油菜, 8个类群为南方油白菜, 及1个混合类群. 我国白菜型油菜的遗传多样性是非常丰富的; 北方小油菜品种之间的遗传差异很大, 春

Genetic Diversity of Germplasm Resources of Brassica campestris L. in China by RAPD Markers

RAPDs (Randomly Amplified Polymorphic DNAs) were used to research the genetic diversity among 172 landraces of Brassica campestris selected from all of China. Forty three random 10 mer primers were tested and 248 polymorphic bands were amplified. By using the dendrogram analysis, 172 varieties were separated into two major groups at the DNA level: 8 vegetable campestris and 164 oil purpose campestris. This result was corresponded to morphological classification and previous conclusions. Vegetable B.campestris was very different from oil purpose B.campestris. Moreover by UPGMA (Un weighted Pair Group Mathematics Average) clustering, the germplasms of 164 oil purpose B. campestris were separated into 15 groups: 6 groups from northern origins, 8 groups are southern origins and one mixed group. The result indicated that the group dividing was related to geographical origins. The diversity level of spring type is higher than that of winter type. The genetic diversity of northern B. campestris was related closely to geographical regions. Spring type campestris was closer to winter type campestris than to semi winter type campestris in genetic relationships and had more extensive difference in genetic diversity than winter type campestris. Semi winter type campestris had a great many genetic variations and its genetic diversity level was also related to geographical origins. Furthermore, landraces from a specific area such as Yunnan Guizhou Hubei provinces and Qinghai Gansu Xinjiang Provinces show more genetic variations and exhibited higher level of genetic diversity than landraces from other provinces, which might give some evidences about origins of B. campestris, as China is the origin center of B. campestris L.