| 甘蓝型油菜显性细胞核雄性不育基因的AFLP标记 |
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| 引用本文： | 陆光远,杨光圣,傅廷栋.甘蓝型油菜显性细胞核雄性不育基因的AFLP标记[J].作物学报,2004,30(2):104-107. |
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| 作者姓名： | 陆光远 杨光圣 傅廷栋 |
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| 作者单位： | 华中农业大学 作物遗传改良国家重点实验室; 国家油菜武汉改良分中心, 湖北武汉 430070 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金，湖北省自然科学基金，高等学校博士学科点专项科研项目 |
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| 摘 要： | 用甘蓝型油菜双基因显性细胞核雄性不育系Rs1046A和欧洲油菜品种Samourai构建了一个回交分离群体。在群分法（BSA）构建的不育池和可育池中共筛选了256对AFLP引物组合，找到了与不育基因紧密连锁的两个AFLP标记(EA03MC1599和EA07MC01235), 它们与不育基因的遗传图距分别是3.5 cM和5.5 cM，而且位于不育基因的同一侧，标记间相
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| 关 键 词： | 甘蓝型油菜 显性细胞核雄性不育基因 AFLP标记 |
| 收稿时间： | 2002-12-02 |
| 修稿时间： | 2002年12月2日 |
Identification of AFLP Markers Linked to the Dominant Genic Male Sterility Gene in Brassica napus L. |
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| LU Guang-Yuan,YANG Guang-Sheng,FU Ting-Dong.Identification of AFLP Markers Linked to the Dominant Genic Male Sterility Gene in Brassica napus L.[J].Acta Agronomica Sinica,2004,30(2):104-107. |
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| Authors: | LU Guang-Yuan YANG Guang-Sheng FU Ting-Dong |
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| Institution: | National Key Lab of Crop Genetic Improvement; National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, Hubei |
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| Abstract: | One backcross population was constructed from the dominant genic male sterile line Rs1046A in Brassica napus L. and the rapeseed cultivar Samourai. Sterile bulk(BS) and fertile bulk(BF) prepared from above population using BSA strategy were subjected to AFLP analysis. A total number of 256 primer combinations were used and two markers(EA03MC15_ 99 and EA07MC01_ 235 ) tightly linked to the dominant genic male-sterile gene( Ms ) were identified, with a map distance of 3.5 cM and 5.5 cM, respectively. These two markers, 2.0 cM away from each other and residing at the same side of Ms, would play an important role in the genetic improvement of this GMS line through marker aided selection(MAS). |
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| Keywords: | Brassica napus L Dominant genic male sterility gene (Ms) AFLP markers |
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