甘薯胚性细胞悬浮培养系的建立

地甘薯胚性细胞悬浮增减系的进行了研究。将１２个基因的长约０．５ｍｍ的茎尖培养在含有０．２ｍｇ／Ｌ或２．０ｍｇ／Ｌ２，４－Ｄ的ＭＳ培养基上，形成了胚性愈伤组织。胚性愈伤组织的形成率因基因型和２，４－Ｄ深度不同而很大差异，为０－７５．７％。一方面，将胚性愈伤组织继续增减在含有２，４－Ｄ的ＭＳ培养基上，它们形成了处于各发育时期的体细胞胚。将具有体细胞胚的胚性愈伤组织转移到ＭＳ基本培养基上，体细胞胚发育成

Establishment of Embryogenic Cell Suspension Cultures in Sweet Potato, Ipomoea batatas(L.)Lam.

Establishment of embryogenic cell suspension cultures in sweet potato was studied. Shoot tips(about 0. 5 mm in length)of 12 genotypes formed embryogenic callus on Murashige and Skoog (MS) medium supplemented with 0. 2 mg/L. or 2. 0 mg/L 2, 4-dichlorophenoxyacetic acid(2,4-D). The frequencies of embryogenic callus formation markedly differed with genotypes and 2, 4-D concentrations, ranging from 0% to 75. 7%. On the one hand, embryogenic calli were continously cultured on MS medium supplemented with 2, 4-1) and produced somatic embryos at various developmental stages. After the embryogenic calli with somatic embryos were transferred to the basal medium, somatic embryos developed into plantlets. The frequencies of embryogenic callus forming plantlets ranged from 5. 9% to 56. 4%. And on the other hand, eight-week-old embryogenic calli of Kokei No. 14 and Xindazi were used to initiate cell suspension cultures. Rapidly-growing and well-dispersed regenerable cell suspension cultures were established. The frequency of somatic embryogenesis from the embryogenic suspension cultures which were maintained for-17 weeks in liquid MS medium containing 2. 0 mg/L 2, 4-D reached 100. 0% in Kokei No. 14 and 53. 6% in Xindazi, respectively, when cell aggregates (about 1 mm in diameter)were transferred to solid MS medium supplemented with 2. 0 mg/L 2, 4-D and further to 1. 0 mg/L abscisic acid(ABA)and the basal medium. Large numbers of plants were regenerated from the embryogenic cell suspension cultures. No morphological variations were observed in the regenerated plants. Such embryogenic cell suspension culture will provide ideal donors for induced somatic mutation, somatic hybridization, and genetic transformation.