| 玉米雄性不育基因(ms30)的RFLP作图 |
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| 引用本文： | 梁业红,周洪生,蒋琬茹.玉米雄性不育基因(ms30)的RFLP作图[J].作物学报,2000,26(3):266-270. |
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| 作者姓名： | 梁业红 周洪生 蒋琬茹 |
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| 作者单位： | 中国农业科学院作物育种栽培研究所， 北京， 100081 |
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| 摘 要： | 以姊妹交第5代群体(SIB5)和回交一代群体(BC1)为作图群体， 对经细胞学初步定位于玉米第4染色体上的雄性不育基因(ms30)进行了RFLP作图。 选用玉米第4染色体上的探针18个， 用集团分离分析(bulked segregatant analysis, BSA)进行标记筛选， 用JoinMap作图软件进行统计分析。 SIB5 群体的RFLP分析表明， ms30基因与玉米第4染
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| 关 键 词： | 玉米 核雄性不育 ms30 BSA RFLP |
| 收稿时间： | 1998-11-07 |
RFLP Mapping of a Male Sterile Gene (ms30) in Maize |
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| LIANG Ye-Hong,ZHOU Hong-Sheng,JIANG Wan-Ru.RFLP Mapping of a Male Sterile Gene (ms30) in Maize[J].Acta Agronomica Sinica,2000,26(3):266-270. |
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| Authors: | LIANG Ye-Hong ZHOU Hong-Sheng JIANG Wan-Ru |
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| Institution: | Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing, 100081 |
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| Abstract: | A sibling population SIB5 and backcross population BC1 were applied to map a male sterile gene ms30 which was early located on chromosome 4 of maize by B-A system. 18 probes from maize chromosome 4 were used, and BSA was practised to screen on RFLPs. By using JoinMap software, linkage as well as ge |
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| Keywords: | Maize Nuclear male sterility ms30 BSA RFLP |
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