| 农杆菌介导法获得小麦转基因植株的研究 |
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| 引用本文： | 黄益洪,周淼平,叶兴国,唐克轩,程红梅,陆维忠.农杆菌介导法获得小麦转基因植株的研究[J].作物学报,2002,28(4):510-515. |
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| 作者姓名： | 黄益洪 周淼平 叶兴国 唐克轩 程红梅 陆维忠 |
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| 作者单位： | 江苏省农业科学院农业生物遗传生理研究所,江苏南京,210014 |
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| 基金项目： | 国家“8 63”(HB-0 2 -0 3 -0 1)资助项目，国家转基因植物专项 (ZJY-A-0 7)项目 |
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| 摘 要： | 从gus基因的瞬时表达入手, 利用不同种类的农杆菌菌株感染小麦不同基因型和同一基因型的不同外植体, 研究了农杆菌菌系、小麦基因型和外植体对转化效率的影响. 从中优选出了对小麦愈伤组织感染力比较强的农杆菌菌系AGL0和MOG101, 以及高敏感受体基因型Alondra和扬麦158等. 实验结果还表明, 预培养10～15天的幼胚愈伤组织具有
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| 关 键 词： | 小麦 农杆菌 基因转化 gus 瞬时麦达 PPT |
| 收稿时间： | 2001-03-16 |
| 修稿时间： | 2001年3月16日 |
Study on the Development of Transgenic Wheat Mediated by Agrobacterium tumefaciens |
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| HUANG Yi Hong,ZHOU Miao Ping,YE Xing Guo,TANG Ke Xuan,CHENG Hong Mei,LU Wei Zhong.Study on the Development of Transgenic Wheat Mediated by Agrobacterium tumefaciens[J].Acta Agronomica Sinica,2002,28(4):510-515. |
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| Authors: | HUANG Yi Hong ZHOU Miao Ping YE Xing Guo TANG Ke Xuan CHENG Hong Mei LU Wei Zhong |
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| Institution: | HUANG Yi Hong 1 ZHOU Miao Ping 1 YE Xing Guo 2 TANG Ke Xuan 3 CHENG Hong Mei 4 LU Wei Zhong 1 |
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| Abstract: | A study on Agrobacterium tumefaciens mediated transformation of wheat was performed by using six Agrobacterium tumefaciens strains, different wheat genetypes, different explants and recombinant plasmids pCAMBIA3301, which contain gus and bar genes. Two highly infectious Agrobacterium tumefaciens strains to wheat and some highly sensitive wheat acceptors were obtained. After cocultivation and two steps selection on a media with herbicides wipeout(20 mg/L) and PPT(3.75 mg/L) respectively, 70 plantlets with PPT resistance were obtained. some of them proved transgenic plants by molecular analysis. |
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| Keywords: | Wheat Agrobacterium tumefaciens Transformation gus PPT Transient expression |
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