绿豆种质资源成株期抗旱性鉴定

作物种质资源抗旱性鉴定是获得抗旱资源和挖掘抗旱基因的前提。本研究以21份绿豆种质资源为材料,采用温室内盆栽控水旱胁迫,考察不同品种单株产量、单株荚重、单株粒数、单株地上部生物量、单株生物量、根冠比等12项指标,计算各指标在旱胁迫与对照条件下的比值,运用相关性分析、隶属函数、抗旱系数和抗旱指数方法,评价绿豆成株期抗旱性,筛选抗旱优异种质。结果表明,抗旱系数与单株地上部生物量、单株总生物量、单株荚重、单株粒数、单株有效荚数在旱胁迫与对照条件下的比值呈极显著正相关,与根冠比呈极显著负相关,从而遴选出这7项指标作为绿豆成株期抗旱性鉴定的评价指标;基于隶属函数、抗旱系数和抗旱指数三者呈极显著正相关,且3种评价抗旱分级结果一致性较高,故认为抗旱指数法适宜于大规模绿豆成株期抗旱性鉴定;筛选获得高抗种质3份、抗旱种质6份、中抗种质4份、敏感种质5份和极敏感种质3份。

Identification of Mungbean Germplasm Resources Resistant to Drought at Adult Stage

Identification of drought resistance is the basic step for selecting drought resistance germplasm resources and mining the drought resistance genes. In this study, 21 mungbean accessions were identified for drought resistance in the different irrigation conditions of greenhouse. Twelve parameters were investigated, including yield per plant, pod weight per plant, seed number per plant, shoot biomass per plant, biomass per plant, root shoot ratio. The ratios of all parameters under the conditions between drought and control were calculated. The identification indices, methods and mungbean germplasm for drought-resistance were evaluated by correlation analysis, subordinative function analysis, drought resistance coefficient and drought resistance index. The results indicated that drought-resistant coefficient had significantly positive correlations with the ratios under the conditions between drought and control for shoot biomass per plant, biomass per plant, pod weight per plant, seed number per plant, effective pod number per plant and significantly negative correlation with that radio for root shoot ratio. The seven indices were recommended for drought resistance identification in mungbean at adult stage. The correlations were significant among parameter values of three evaluation methods, with a very high consistency. Drought resistance index was recommended to screen a large number of germplasm resources of mungbean at adult stage. The various resistant accessions of mungbean germplasm resources were screened by drought resistance index including three varieties with high resistance, six with resistance, four with moderate resistance, five with susceptibility, and three with high susceptibility.