分子标记辅助选择的修饰回交聚合育种方法及其在棉花上的应用

修饰回交育种法是将杂种品系间杂交和回交方法结合起来，用于棉花多个优良性状聚合的育种改良方法。随着分子标记技术日益完善地用于育种的选择中，我们提出了分子标记辅助选择的修饰回交聚合育种方法。它以生产上推广或即将推广的品种为轮回亲本，将修饰回交育种法和分子标记辅助选择育种相结合，同时对轮回亲本的遗传背景和

Modified Backcross Pyramiding Breeding with Molecular Marker-Assisted Selection and Its Applications in Cotton

Using upland cotton (G.hirsutum L.) as research material,our objective was to describe and implement a modified backcrossing approach for introgressing transgenes or quantitative trait loci (QTLs) into target genetic backgrounds. Modified backcrossing is an improved breeding procedure combining inter-strain crossing and backcrossing methods to pyramid many traits. In the wake of availability of molecular marker technology for application to plant breeding, we propose a new backcrossing method called modified backcross pyramiding breeding (MBPB) combined with molecular marker-assisted selection (MAS). With MBPB, MAS was used in modified backcrossing and selection, i.e., target genes or QTLs and the recurrent parental background was simultaneously selected by molecular markers.Henceforth, the breeding efficiency was significantly improved.By using this procedure, QTLs for stronger fiber strength and transgene cryIA have been rapidly pyramided in new cultivar NAU 85188 with Simian 3 background in cotton. The new cotton cultivar is characterized by elite fiber qualities, insect-resistance and high yield potential.The described breeding procedure can be used to simultaneously pyramid transgenes or QTLs in modern breeding programs.