| 中国江、浙地区栽培大麦遗传资源的RAPD研究 |
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| 引用本文： | 施永泰,边红武,韩凝,潘建伟,童微星,朱睦元.中国江、浙地区栽培大麦遗传资源的RAPD研究[J].作物学报,2004,30(3):258-265. |
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| 作者姓名： | 施永泰 边红武 韩凝 潘建伟 童微星 朱睦元 |
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| 作者单位： | 浙江大学生命科学学院, 浙江杭州310012 |
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| 基金项目： | 国家自然科学基金，浙江省科研项目，国家高技术研究发展计划(863计划) |
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| 摘 要： | 采用随机扩增多态性（RAPD）标记，探讨了我国部分栽培大麦品种的遗传背景。结果表明：200个随机引物中，有30个引物扩增出的产物具有多态性，30个引物共扩增出223条谱带，其中130条谱带具有多态性，每个引物可扩增出2~9条多态性谱带，平均4.19条。67个大麦品种平均表型多样性值0.369，裸麦的表型多样性高于皮麦的表型多样性
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| 关 键 词： | 栽培大麦 RAPD 遗传多样性 聚类分析 |
| 收稿时间： | 2002-08-22 |
| 修稿时间： | 2002年8月22日 |
Genetic Variation Analysis by RAPD of Some Barley Cultivars in China |
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| SHI Yong-Tai,ZHU Mu-Yuan.Genetic Variation Analysis by RAPD of Some Barley Cultivars in China[J].Acta Agronomica Sinica,2004,30(3):258-265. |
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| Authors: | SHI Yong-Tai ZHU Mu-Yuan |
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| Institution: | College of Life Sciences, Zhejiang University, Hangzhou 310012, Zhejiang;2.Ningbo Academy of Agricultural Science, Ningbo 315040, Zhejiang |
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| Abstract: | Genetic variation is essential for genetic improvement and plant breeding. 200 arbitrary primers (10-mers) were used for the PCR amplification of random genetic DNA fragments in this study to estimate the genetic variation of 67 barley cultivars in China by RAPD, most of which were derived from the Zhejiang-Jiangsu planting zone. In total 223 bands or 130 polymorphic bands were amplified by 30 primers. Each primer could amplify 2 to 9 polymorphic bands, with an average of 4.19 bands. The value of phenotypic diversity in naked barley cultivars was higher than that of hulled barley cultivars. The lowest phenotypic diversity value was found in 2-rowed hulled barley cultivars. Cluster analysis showed that the 67 cultivars could be classified into 4 groups at the level of D 0.6, which evidently showed some variation of some important traits of barley. |
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| Keywords: | RAPD |
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