延缓衰老基因PPF1转化水稻的初步研究

利用农杆菌介导法，把短日豌豆花后特异表达、具有延缓衰老作用的基因PPF1（post-floral-specific gene expressed in short-day-grown G2 pea）转入水稻，以期获得延缓衰老的转基因植株。约970块未成熟胚性愈伤组织与农杆菌共培养后，得到了约280块抗性愈伤组织，从中分化出抗性绿苗54株。PCR和Southern blotting检测结果表

Preliminary Study on Introducing of Leaf Senescence-Delaying Gene PPF1 into Rice

Premature senescence of leaf, usually results in too much abortive grains and lowing in seed-setting-rate, is a common problem for many plants and always led to great loss of production. In this study, the leaf senescence-delaying gene, PPF1, a post-floral-specific gene expressed in short-day-grown G2 pea,was introduced into rice to delay the progress of leaf senescence by the approach of Agrobacterium-mediated transformation. After the co-cultivation of over 970 scutellum calli with Agrobacterium tumefaciens, about 280 hygromycin-resistant calli were obtained, and 54 independent plantlets derived from different calli were regenerated from them(Table 1). The results from PCR (Fig.2, 3) and Southern blotting(Fig.4) demonstrated that the target gene was integrated into the genome of 42 plantlets with a positive ratio of 77.7%. The chlorophyll content, photosynthetic index and leaf senescence in T_0 transgenic plants were also discussed. The results indicated that most of the transgenic plants were remarkably higher than the control both in the chlorophyll content(Fig.5) and photosynthetic index(Fig.6), and as a result, the leaf senescence process in the transgenic plants was obviously inhibited(Table 2). It suggested that PPF1 gene might express in the transgenic plants, and the leaf senescence was delayed, which may show a new way to improve the rice production.