两个谷子CIPK基因在非生物逆境胁迫下的表达分析

CIPK是一类丝氨酸/苏氨酸蛋白激酶,该蛋白在植物响应逆境胁迫过程中发挥重要作用。本文利用生物信息学方法从谷子基因组中鉴定出2个CIPK基因,命名为Si CIPK6和Si CIPK16。序列分析表明Si CIPK6基因组序列长1994 bp,编码451个氨基酸;Si CIPK16基因组序列长1885 bp,编码473个氨基酸,2个基因均无内含子和可变剪切。生物信息学分析显示这2个基因在蛋白质序列和结构上与其他物种CIPK基因一样非常保守。实时定量PCR分析表明Si CIPK6和Si CIPK16在ABA、低温、高温、干旱、高盐诱导下表达量均有所上调,Si CIPK6基因在ABA、干旱和盐处理时表达量上调幅度较大,而Si CIPK16基因在低温、干旱和高温处理时表达量上调幅度较大。半定量PCR检测结果表明Si CIPK6和Si CIPK16两个基因在拔节、孕穗、灌浆期时均有表达,在相应生育时期受到干旱胁迫时它们表达量均有所提高。推测Si CIPK6和Si CIPK16基因在谷子的干旱或其他逆境胁迫中起一定作用。

Expression Analysis of Two CIPK genes under Abiotic Stress in Foxtail Millet

CIPK (CBL interacting protein kinase) is a type of serine or threonine protein kinases, which plays an important role in response to stress. In this study, we identified two CIPK genes designated as SiCIPK6 and SiCIPK16 from foxtail millet (Setaria italica) genome using bioinformatics methods. The sequence analysis showed that SiCIPK6 has a length of 1994 bp in the genome, encoding 513 amino acids residues, and SiCIPK16 is 1885 bp, encoding 473 amino acids residues. These two genes have no alternative splicing and intron. The characters predicted based on the bioinformatics analysis revealed that the protein sequences and structure of the two SiCIPK genes were very conservative just like CIPK genes in other species. Real-time PCR analysis discovered that the expression of SiCIPK6 and SiCIPK16 was up-regulated by ABA, cold, heat, drought and salt stress, respectively. The expression was strongly induced by ABA, drought and salt treatments for SiCIPK6, and by cold, drought and heat treatments for SiCIPK16. The semi-quantitative PCR analysis showed that SiCIPK6 and SiCIPK16 were expressed at the jointing, booting and filling stages, and induced by drought stress in the corresponding growth period. Foxtail millet CIPK genes reported in this study would enrich CIPK members in plant kingdom and provides important information for further elucidating the function and mechanisms of the CBL/CIPK network system responsing to stresses in foxtail millet.