首页 | 本学科首页   官方微博 | 高级检索  
     检索      

农杆菌介导NHX基因转化甘蓝型油菜的研究
引用本文:王艳,曾幼玲,贺宾,秦丽,李金耀,高燕,张富春.农杆菌介导NHX基因转化甘蓝型油菜的研究[J].作物学报,2006,32(2):278-282.
作者姓名:王艳  曾幼玲  贺宾  秦丽  李金耀  高燕  张富春
作者单位:新疆生物资源基因工程重点实验室, 新疆大学生命科学与技术学院,新疆乌鲁木齐830046
基金项目:国家科技攻关项目;科技部专项基金;教育部春晖计划项目
摘    要:以甘蓝型油菜带柄子叶为转化受体,通过根癌农杆菌EHA105(Agrobacterium tumefaciens)介导将盐地碱蓬(Suaeda heteroptera)的Na+/H+反向运输体基因NHX导入新疆主栽油菜1khp11品系中,获得了抗卡那霉素的再生植株,并对影响遗传转化的一些关键因素进行了研究。结果表明,带柄子叶在含有2,4-D的培养基上经过3 d预培养后于浓度OD600为0.3~0.4的菌液中浸染7 min, 卡那抗性绿苗率可达8%~9%,经过对抗性植株的PCR检测、点杂交及PCR-Southern杂交证明外源基因已整合到油菜基因组中,为新疆盐碱土上油菜产量的提高提供了新的途径。

关 键 词:根癌农杆菌  带柄子叶  预培养  菌液浓度  浸染时间  转基因油菜
收稿时间:2004-12-28
修稿时间:2005-08-16

Research of NHX Gene Transformation in Brassica napus by Agrobacterium tumefaciens
WANG Yan,ZENG You-Ling,HE Bin,QIN Li,LI Jin-Yao,GAO Yan,ZHANG Fu-Chun.Research of NHX Gene Transformation in Brassica napus by Agrobacterium tumefaciens[J].Acta Agronomica Sinica,2006,32(2):278-282.
Authors:WANG Yan  ZENG You-Ling  HE Bin  QIN Li  LI Jin-Yao  GAO Yan  ZHANG Fu-Chun
Institution:Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology, Xinjiang University,Urumqi 830046, Xinjiang
Abstract:The crop yield is affected seriously by the salty soil in Xinjiang.Cotyledons with petiole of the line of Brassica napus 1khp11 were transformed with strain of Agrobacterium tumefaciens EHA105,harboring Na~ /H~ antiporter gene of Suaeda heteroptera in order to select the salt tolerant oilseed.The regeneratedly kan-resistant plants were obtained.In the research,major factors of transformation were discussed detailedly.The results showed that the best physiological and transforming condition was preculture for 3 days with 1 mg/L 2,4-D for cotyledons with petiole and immersion in OD_(600) 0.3(0.4 liquid bacterium) for 7 min,8%9% kan-resistant green seedlings were obtained.It was proved that NHX gene was inserted into the genome of oilseed by the methods of PCR,Dot blot and PCR-Southern.
Keywords:Agrobacterium tumefaciens  Cotyledons with petiole  Preculture  Concentration of bacterium  Immerged time  Transgenic oilseed
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《作物学报》浏览原始摘要信息
点击此处可从《作物学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号