3种接种方法对十字花科黑腐病菌Xcc8004菌株在拟南芥Col-0上致病力的影响

十字花科黑腐病菌（Xanthomonas campestris pathovar carnpestris，Xcc）是引起十字花科植物黑腐病的病原菌，也是研究寄主与病原微生物相互作用分子机理的模式菌之一。Xcc可以感染白菜、萝卜和甘蓝等十字花科农作物，也可以感染重要的模式植物拟南芥（AroJoidopsisthaliana）。由于拟南芥的全基因组测序已经完成，因此，拟南芥是研究寄主植物对Xcc浸染的防卫反应的分子机理的最理想的寄主材料。但是，到现在为止，一套完善的在拟南芥上进行xcc致病检测的实验系统还没有建立。为此，本研究比较了“叶片压渗法”、“剪叶法”和“叶片中脉穿刺法”这3种常用的病原细菌接种方法对Xcc的实验室菌株8004f以下简称Xcc8004）在哥伦比亚生态型拟南芥似．thanlianaecoype Colombia0，Col-0）上的致病力的影响。结果发现，在拟南芥Col-0叶片上用“叶片压渗法”接种Xcc8004可以引起明显致病症状，而用“剪叶法”和“叶片中脉穿刺法”接种均不能引起病症。这一结果说明，不同的接种方法的对Xcc在拟南芥上的致病力有很大的影响。因此，要在拟南芥Col-0上进行Xcc8004的致病力检测，本研究建议采用“叶片压渗法”而不用“剪叶法’和“叶片中脉穿刺法”。此外，本研究还建立了一套简易的拟南芥试验用苗的栽培方法。

Effect of 3 Inoculation Methods on the Virulence ofXanthomonas campestris pathovar campestris strain 8004 on A rab idops is thaliana Col-0

Xanthomonas campestris pathovar campestris （Xcc） is the causal agent of black rot disease ofcruciferous crops, and is a model strain for studying the molecular mechanisms of plant-microbe interactions. Xcc infects almost all the members of crucifer family （Brclssicaceae） such as cabbage, radish and cauliflower, and the model plant A r- abidopsis thaliana. Since the whole genome ofArabidopsis thaliana has been sequenced, which has became the best host plant for studying the molecular basis for the host defense against Xcc. However, the method for testing the pathogenicity of Xcc on A rabidopsis thaliana has not been well established so far. For this, in this study, the re- sponse of the wild typeA rabidopsis thaliana ecoype Colombia 0 （Col-0） to the infection byXcc strain 8004 （Xcc8004） was respectively tested by using leaf-infiltration, leaf-clipping and leaf main vein-piercing method. The results show that Xcc8004 can cause disease on the leaf of A rabidopsis thaliana Col-0 by leaf-infiltration, but not by leaf-clipping or leaf central vein-piercing. These results reveal that the pathogenicity of Xcc 8004 in Arabidopsis is strongly affected by the inoculation method used, and leaf-infiltration is a suitable method but leaf-clipping or leaf central vein-piercing is not a suitable method for which. In addition, a simple and efficient Arabidopsis thaliana planting method was established in this study.