我国南方甘蔗病毒种类初步鉴定

依据G enB ank中已报道的甘蔗主要病毒基因组序列设计PCR引物,以采自我国广东、广西、云南、海南等地的甘蔗叶组织总RNA或总DNA抽提物为模板,采用一步法RT-PCR及PCR扩增,对预期扩增产物克隆、测序,根据序列同一性判断测定样品是否含有预期病毒,结果表明,我国南方甘蔗已受到甘蔗花叶病毒(Sugarcane m osa ic v irus,SCM V)、高粱花叶病毒(Sorghum m osa ic v irus,S rM V)、甘蔗黄叶病毒(Sugarcane ye llow leaf v irus,SCYLV)及甘蔗杆状病毒(Sugarcane B ac illiform V irus,SCBV)的侵染,未发现甘蔗线条花叶病毒(Sugarcane streak m osa ic v irus,SCSM V)及甘蔗线条病毒(Sugarcane streak v irus,SSV)。

Preliminary identification of sugarcane viruses occurred in south China

RT-PCR and PCR were performed with primers based on reported sugarcane viral genomic sequence in GenBank database and total RNA or DNA extracted from sugarcane leaf tissue collected from south China including Guangdong,Guangxi,Yunnan,Hainan provinces.Amplified DNA products with expected size were cloned and sequenced.Virus was identified according to the nucleotide identities with reported viral genomic sequence.It was revealed that the sugarcane grown in south China was infected with Sugarcane mosaic virus(SCMV),Sorghum mosaic virus(SrMV),Sugarcane yellow leaf virus(SCYLV) and Sugarcane Bacilliform Virus(SCBV);but Sugarcane streak mosaic virus(SCSMV) and Sugarcane streak virus(SSV)did not be found.