| 青钱柳种质资源多样性SRAP初步分析 |
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| 引用本文： | 周一旸,洑香香,尚旭岚,杨万霞,方升佐.青钱柳种质资源多样性SRAP初步分析[J].广西农业生物科学,2011,30(1):40-46. |
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| 作者姓名： | 周一旸 洑香香 尚旭岚 杨万霞 方升佐 |
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| 作者单位： | 南京林业大学森林资源与环境学院,南京,210037 |
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| 基金项目： | 国家林业局林业公益性行业科研专项(200904046); 江苏省“六大人才高峰”资助项目“珍贵青钱柳种质资源保存及其开发利用技术研究”; 江苏高等学校创新团队科研计划; “教育部质量工程”国家级林学实验示范中心和“人才培养模式创新实验区——林学专业人才培养模式创新”项目共同资助 |
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| 摘 要： | 为了利用分子标记方法评价青钱柳种质资源的遗传多样性,本文对青钱柳DNA的提取、SRAP扩增体系重要参数进行了优化,运用优化体系筛选多态性引物,并用1对引物对青钱柳9个种源进行了遗传多样性初步分析。研究结果建立了适于青钱柳SRAP的扩增体系;从110对SRAP引物中筛选出了13对多态性引物,运用1对引物组合Me7＋Em2扩增获得21个多态性位点,多态率达100%。遗传多样性分析表明有效等位基因数（Ne）为1.3429,平均Shannon＇s信息指数（I）为0.3687,Nei＇s基因多样性指数（H）为0.2267,种源的遗传分化指数Gst为0.1983;聚类分析结果表明9个种源在遗传距离0.100处聚为3类,聚类结果和地理距离之间呈现较高的相关性。本文的研究结果表明所建立的青钱柳种质资源库具有广泛的遗传多样性,为进一步的开发利用提供了条件。
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| 关 键 词： | 青钱柳 SRAP 优化 遗传多样性 聚类 |
Preliminary Study on the Genetic Diversity of Germplasm for Cyclocarya paliurus Revealed by SRAP Markers |
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| Zhou Yiyang Fu Xiangxiang Shang Xulan Yang Wanxia Fang Shengzuo College of Forest Resources , Environment,Nanjing Forestry University,Nanjing.Preliminary Study on the Genetic Diversity of Germplasm for Cyclocarya paliurus Revealed by SRAP Markers[J].Journal of Guangxi Agricultural and Biological Science,2011,30(1):40-46. |
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| Authors: | Zhou Yiyang Fu Xiangxiang Shang Xulan Yang Wanxia Fang Shengzuo College of Forest Resources Environment Nanjing Forestry University Nanjing |
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| Institution: | Zhou Yiyang Fu Xiangxiang * Shang Xulan Yang Wanxia Fang Shengzuo College of Forest Resources and Environment,Nanjing Forestry University,Nanjing,210037 |
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| Abstract: | In order to assess the genetic diversity of germplasm for Cyclocarya paliurus by molecular markers, DNA extraction method and key parameters of SRAP reaction system were optimized; polymorphic primers were screened with the optimal system, and preliminary analysis of genetic diversity of 9 provenances by using a pair of primers was operated in this paper. The results showed that a SRAP reaction system for Cyclocarya paliurus was established through optimal design. 13 pairs of polymorphic primers were screen... |
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| Keywords: | Cylocarya paliurus SRAP Optimization Genetic polymorphism Cluster |
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