| 多选择标记植物表达载体的构建 |
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| 引用本文： | 李春艳,吴三民,赵建宇,樊宪伟,李有志.多选择标记植物表达载体的构建[J].广西农业生物科学,2011,30(1):27-34. |
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| 作者姓名： | 李春艳 吴三民 赵建宇 樊宪伟 李有志 |
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| 作者单位： | 广西亚热带生物资源保护利用重点实验室,南宁,530005 |
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| 基金项目： | 973计划项目“农作物骨干亲本农艺性状基因与环境互作研究(编号:2006CB101706)”; “广西科学研究与技术开发项目(桂科攻10100005-4)”共同资助 |
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| 摘 要： | 具有多选择标记的植物基因表达载体有利于转基因植物研究中的转基因植株的筛选。本研究对植物基因表达载体pCAMBIA1301进行了改造,产生了1个具有可溶性的红移绿色荧光蛋白基因（smRS-GFP）、抗除草剂Basta、葡萄糖苷酸酶（GUS）及潮霉素（Hpt）的多选择标记的新的植物基因表达载体。运用这一表达载体的多选择标记可以有效降低检测和筛选转化植株时的假阳性率。此外,如此的基因表达载体也能满足实验室的不同筛选方法的需求。
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| 关 键 词： | 多选择标记 红移绿色荧光蛋白 植物表达载体 |
Construction of Plant Expression Vectors with Multi-selectable Marker |
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| Li Chunyan , Wu Sanmin , Zhao Jianyu , Fan Xianwei , Li Youzhi , Guangxi Key Laboratory of Subtropical Bioresource Conservation , Utilization,Nanning, College of Life Science , Technology,Guangxi University, The Key Laboratory of Ministry of Education of China for Microbial , Plant Genetic Engineering.Construction of Plant Expression Vectors with Multi-selectable Marker[J].Journal of Guangxi Agricultural and Biological Science,2011,30(1):27-34. |
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| Authors: | Li Chunyan Wu Sanmin Zhao Jianyu Fan Xianwei Li Youzhi Guangxi Key Laboratory of Subtropical Bioresource Conservation Utilization Nanning College of Life Science Technology Guangxi University The Key Laboratory of Ministry of Education of China for Microbial Plant Genetic Engineering |
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| Institution: | Li Chunyan 1,2,3 Wu Sanmin 1,3 Zhao Jianyu 1,3 Fan Xianwei 1,3* Li Youzhi 1,3* 1 Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization,Nanning,530005,2 College of Life Science and Technology,Guangxi University,3 The Key Laboratory of Ministry of Education of China for Microbial and Plant Genetic Engineering |
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| Abstract: | A plant gene expression vector with multi-selectable markers benefits screening of transgenic plants in plant transgene researches. In this study, a plant gene expression vector pCAMBIA1301 was re-constructed, generating a new plant expression vector with multi-selectable markers of red shift green fluorescent protein (smRS-GFP) gene, herbicide Basta, and hygromycin (Hpt). Use of multi-selectable markers of the expression vector would efficiently reduce false positive rate in detecting and screening of tran... |
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| Keywords: | Multi-selectable marker Red shift green fluorescent protein Plant expression vector |
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