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水杨酸诱导下人参培养物差异表达基因片段的克隆
引用本文:张悦,王义,蒋世翠,张明哲,李凤华,张美萍.水杨酸诱导下人参培养物差异表达基因片段的克隆[J].广西农业生物科学,2009(2):245-250.
作者姓名:张悦  王义  蒋世翠  张明哲  李凤华  张美萍
作者单位:吉林农业大学生命科学学院;黑龙江广播电视大学;
基金项目:吉林省科技发展计划项目(20051113);;吉林省科技厅吉林省科技发展计划项目(20060546);;吉林省中医药管理局资助项目(2004116);;吉林农业大学青年启动基金以及吉林农业大学博士启动基金共同资助
摘    要:本研究以人参愈伤悬浮细胞为材料,在其生长的第28天添加1×10^-3mg/L水杨酸,测定水杨酸添加后,过氧化物酶、多酚氧化酶和苯丙氨酸解氨酶等3种酶在72h内的变化及皂苷含量,结果表明:水杨酸添加后对过氧化物酶和苯丙氨酸解氨酶的活力影响最大,分别在24h和48h达到最大峰值,在18h开始影响多酚氧化酶的活力,培养物生长的第28天添加水杨酸可以明显提高人参愈伤组织中皂苷的合成。确定添加水杨酸后24h提取总RNA,进行cDNA-RDA分析,筛选差异片段。确定差异基因并在GenBank中注册,注册号为FE900130。为探讨水杨酸作为诱导子对人参次生代谢的影响奠定基础。

关 键 词:人参  水杨酸诱导子  cDNA-RDA  差异表达基因

Cloning of the Differential Expression Fragment from Ginseng Culture Induced by Salicylic Acid
Zhang Yue Wang Yi Jiang Shicui Zhang Mingzhe Li Fenghua Zhang Meiping College of Life Science,Jilin Agricultural University,Changchun, Heilongjiang Radio , TV University,Harbin.Cloning of the Differential Expression Fragment from Ginseng Culture Induced by Salicylic Acid[J].Journal of Guangxi Agricultural and Biological Science,2009(2):245-250.
Authors:Zhang Yue Wang Yi Jiang Shicui Zhang Mingzhe Li Fenghua Zhang Meiping College of Life Science  Jilin Agricultural University  Changchun    Heilongjiang Radio  TV University  Harbin
Institution:Zhang Yue 1 Wang Yi 1 Jiang Shicui 1 Zhang Mingzhe 1 Li Fenghua 2 Zhang Meiping 1 1 College of Life Science,Jilin Agricultural University,Changchun,130118,2 Heilongjiang Radio , TV University,Harbin,150000
Abstract:In this reserch, the materials are ginseng callus suspension cells which are supplied with 1×10^-3mg/L salicylic acid on the 28th day of growth. Determination changes of the peroxidase, polyphenol oxidase, phenylalnine ammonialyase in 72 h in the content of saponin after salicylic acid added. The results showed: the peroxidase, phenylalnine ammonialyase most dynamic, 24 h and 48 h respectively reach the largest peak in 18 hours began to affect the vitality of the polyphenol oxidase after salicylic acid added, the growth of the culture section 28 days of salicylic acid can add significantly to raise ginseng callus saponin synthesis. It had been determined that the total RNA should be abstracted after 24 h when Salicylic acid added. The cDNA representational difference analysis (cDNA-RDA) technology was applied to analysis and screening the different fragment. The differential expression was determined and registered in GenBank while the registration number is FE900130. Our work laid the foundation for the study of salicylic acid as an elicitor on the impact of gensing secondary metabolites.
Keywords:Panax ginseng C  A  Meyer  Salicylic acid elicitor  cDNA-RDA  Differential expression gene  
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