| 农杆菌介导的玉米茎尖遗传转化研究及转TPS1基因和VlmybA2基因玉米鉴定 |
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| 引用本文： | 安立昆,赵德刚.农杆菌介导的玉米茎尖遗传转化研究及转TPS1基因和VlmybA2基因玉米鉴定[J].广西农业生物科学,2014(3):578-584. |
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| 作者姓名： | 安立昆 赵德刚 |
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| 作者单位： | 贵州大学生命科学学院山地植物资源保护与种质创新省部共建教育部重点实验室,贵州省农业生物工程重点实验室,贵阳550025 |
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| 基金项目： | 国家转基因生物新品种培育重大科技专项《安全转基因技术研究》子项目任务《保障转基因生物安全的外源基因清除技术研究》编号2014ZX0801008B-002资助 |
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| 摘 要： | 本研究以优良玉米自交系交51为材料,通过农杆菌介导的茎尖转化将海藻糖-6-磷酸合成酶基因TPS1和葡萄花青素调控基因VlmybA2转入玉米中。确立了最佳的除草剂Basta筛选浓度,研究了不同真空渗透压、农杆菌液浓度、侵染时间等因素在转化过程中对玉米植株存活率的影响。结果表明80 mg/L除草剂Basta为最佳筛选浓度,真空渗透压为60 kPa、农杆菌液浓度OD600为0.8、侵染时间为8 min时是最佳转化条件,经过GUS组织化学染色和PCR检测,证明TPS1基因和VlmybA2基因已经整合到玉米基因组中。
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| 关 键 词： | 玉米 茎尖 农杆菌 TPS1基因 VlmybA2基因 |
Agrobacterium-mediated Genetic Transformation of Maize Shoot Apical Meristem and Detection of Transgenic Maize with TPS1 Gene and VlmybA2 Gene |
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| An Likun,Zhao Degang.Agrobacterium-mediated Genetic Transformation of Maize Shoot Apical Meristem and Detection of Transgenic Maize with TPS1 Gene and VlmybA2 Gene[J].Journal of Guangxi Agricultural and Biological Science,2014(3):578-584. |
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| Authors: | An Likun Zhao Degang |
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| Institution: | ( Guizhou Key Lab of Agro-Bioengineering, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, College of Life Sciences, Guizhou University, Guiyang, 55002) |
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| Abstract: | TPS1 gene and VlmybA2 gene were transformed into shoot apical meristem of maize elite inbred line Jiao51 by Agrobacterium-mediated transformation. The research established the optimum concentration of herbicide Basta in selection condition. The effects of plant survival factors in the transformation process of vacuum, osmotic pressure, agrobacterium concentration, infection time, had been studied. The results showed the 80 mg/L Basta was the optimum selection condition. The vacuum osmotic for 60 kPa, agrobacterium concentration for OD600=0.8, infection time for 8 min were the optimum transformation condition. The transgenic plants were obtained after confirmed by GUS staining and PCR amplification. It was proofed that TPS1 gene and VlmybA2 gene has integrated into maize. |
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| Keywords: | Maize（Zea mays L ） Shoot apical Agrobactcrium tumefaciens TPS1 gene VlmybA2 gene |
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