| 纤维素酶高产菌株桧状青霉9-3的诱变选育及产酶条件研究 |
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| 引用本文： | 张粲,;武改红,;赫荣琳,;于俊杰,;马丽娟,;李晨,;贾文娣,;杨洪江,;张东远.纤维素酶高产菌株桧状青霉9-3的诱变选育及产酶条件研究[J].广西农业生物科学,2014(3):564-569. |
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| 作者姓名： | 张粲 ;武改红 ;赫荣琳 ;于俊杰 ;马丽娟 ;李晨 ;贾文娣 ;杨洪江 ;张东远 |
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| 作者单位： | [1]天津科技大学生物工程学院,天津300457; [2]中国科学院天津工业生物技术研究所天津市工业生物系统与过程工程重点实验室,天津300308 |
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| 基金项目： | 高活力葡萄糖苷酶菌株筛选及产酶工艺优化（863课题：No.2012AA023202）资助 |
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| 摘 要： | 本文以桧状青霉9-3为出发菌株,采用硫酸二乙酯（DES）诱变,通过筛选得到一株酶活力高且遗传稳定性良好的菌株H16,其滤纸酶活力、β-葡萄糖苷酶酶活力与蛋白产量均较出发菌株相比均提高了4倍左右。并通过对发酵培养基以及发酵条件的优化,确定了最佳的产酶条件为：微晶纤维素浓度为2%,玉米浆干粉浓度为1.5%,发酵温度为30℃,初始pH为5.5,装液量为30 mL/250 mL,发酵周期为5 d。在优化的条件下,该菌株的纤维素酶和蛋白产量均进一步提高25%左右。
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| 关 键 词： | 桧状青霉 纤维素酶 诱变筛选 酶活 |
Mutagenesis,Screening of Cellulase over Producing Mutant of Penicillium piceum 9-3 and Fermentation Conditions Optimization |
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| Institution: | Zhang Can ,Wu Gaihong , He Rongjie , Yu Junjie , Ma Lijuan , Li Chen , Jia Wendi , Yang Hongjiang,Zhang Dongyuan ( 1 College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457; 2 Tianjin Key Laboratory for Industrial Biological Sys- tems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308) |
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| Abstract: | In this study, a cellulase over producing mutant with genetic stability of Penicillium piceum H16 was obtained through DES mutagenesis. FPA, beta-glucosidase and secreted protein were all improved four folds. An optimal fermentation conditions was obtained using single factor experiment. The optimal factors were： avicel as carbon source, corn steep powder as nitrogen souce, initial pH 5.5, temperature 30 ℃, 30 mL fermentation medium in 250 mL flasks, 5 days cultivation. |
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| Keywords: | Penillium piceum Cellulase Screening Enzyme activity |
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