| 基于Illumina测序技术挖掘Bt杀虫基因资源 |
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| 引用本文： | 张文飞,;吴红萍,;徐志霞,;金映虹,;吴仲琦,;钤江朝,;黄楠楠,;金文杰,;贾璐羽,;栗新峰,;王宁,;赵丛,;刘肖静,;刘洁,;刘明月,;刘义凤,;王锐萍.基于Illumina测序技术挖掘Bt杀虫基因资源[J].广西农业生物科学,2014(3):472-477. |
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| 作者姓名： | 张文飞 ;吴红萍 ;徐志霞 ;金映虹 ;吴仲琦 ;钤江朝 ;黄楠楠 ;金文杰 ;贾璐羽 ;栗新峰 ;王宁 ;赵丛 ;刘肖静 ;刘洁 ;刘明月 ;刘义凤 ;王锐萍 |
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| 作者单位： | [1]热带动植物生态学省部共建教育部重点实验室,海南师范大学生命科学学院,海口571158; [2]海南省热带农业资源研究所,三亚572025 |
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| 基金项目： | 海南省高等学校科学研究项目（Hjkj2013-19）; 海南师范大学大学生创新创业训练计划项目共同资助 |
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| 摘 要： | 苏云金芽孢杆菌作为应用最为广泛的生物农药,已经成为化学合成农药必要的补充或替代品,其杀虫蛋白基因已成功的应用到转基因抗虫作物中。本文基于海南五指山和吊罗山热带原始雨林区土壤样品筛选分离的Bt菌株资源,利用第二代测序技术Illumina对分离的特异Bt菌株的杀虫基因进行全面分析,鉴定出新型Bt杀虫蛋白基因25个,其中包括5个新型的营养期杀虫蛋白基因（vip）和3个新型cyt基因。本研究表明第二代DNA测序技术可以高效用于挖掘Bt杀虫基因资源,而获得的新型杀虫蛋白基因将进一步丰富我国能够应用的杀虫基因种类。
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| 关 键 词： | Illumina测序 苏云金芽孢杆菌 杀虫蛋白 |
Mining New Bt Insecticidal Protein Genes Resource Using Illumina Sequencing Technology |
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| Institution: | Zhang Wenfei,Wu Hongping,Xu Zhixia,Jin Yinghong,Wu Zhongqi,Qian Jiangzhao,Huang Nannan,Jin Wenjie,Jia Luyu,Li Xinfeng,Wang Ning,Zhao Cong,Liu Xiaojing,Liu Jie,Liu Mingyue,Liu Yifeng,Wang Ruiping ( 1 Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou, 571158; 2 Hannah Institude of Tropical Agricultural Resource, Sanya, 572025) |
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| Abstract: | Bacillus thuringiensis（Bt）, as the most widely used biologically produced pest control agent, is already a useful alternative or supplement to synthetic chemical pesticide and is also a key source of genes for transgenic expression to provide pest resistance in plants. On basis of Bt strains isolated from soil samples from the Diaoluoshan and Wuzhishan Natural Reserve, we carried out a large-scale screening the insecticidal genes from the special Bt strains through Illumina sequencing. In this study, 25 of the insecticidal genes, including 5 of the novel vegetative insecticidal protein genes and 3 of the new cyt genes had been identified from the different Bt strains. All the result indicated that the second generation sequencing technology of illumine is efficient in mining the insecticidal gene from Bt isolates, which would further enrich the new different insecticide genes application in commercial agriculture, forest management, and mosquito control. |
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| Keywords: | Illumina sequencing Bacillus thuringiensis Insecticide proteins |
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