| 正常生理及ISCOM疫苗免疫下小鼠乳腺Fc受体基因表达变化 |
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| 引用本文： | 张春林,刘光磊,王加启,卜登攀,赵圣国,周凌云,赵国琦.正常生理及ISCOM疫苗免疫下小鼠乳腺Fc受体基因表达变化[J].广西农业生物科学,2010(1):51-56. |
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| 作者姓名： | 张春林 刘光磊 王加启 卜登攀 赵圣国 周凌云 赵国琦 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室;扬州大学动物科学与技术学院; |
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| 基金项目： | 国家自然科学基金(30871837);;国际科技合作项目(2009DFB30530);;动物营养学国家重点实验室自主研究课题(2004DA125184(青)0801)共同资助 |
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| 摘 要： | 本研究对小鼠不同生理阶段以及免疫条件下,乳腺内FcRn基因mRNA表达量的变化进行了分析。选择100只健康昆明小白鼠分为4组,在其分娩后4d分别进行如下制剂处理：灭菌生理盐水、脂肪酶蛋白（Lipase）＋灭菌生理盐水、空免疫刺激复合物（ISCOM）、Lipase-ISCOM。分娩后8d和12d用间接ELISA法测定乳中和血清中的特异性IgG效价,用RealTimePCR检测小鼠乳腺中FcRn基因的相对表达量,而正常生理状态乳腺内FcRn基因mRNA表达量的变化分别是在分娩后1d、4d、8d和12d进行检测。结果表明：正常状态下,乳腺B2M和FCGRT基因mRNA分别在0和1d表达最高,随后表达量下降并保持在一个稳定水平。免疫条件下,乳腺B2M和FCGRT基因mRNA显著上升。由此可知,免疫刺激可以引起小鼠乳FcRn基因表达上调。
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| 关 键 词： | 小鼠 免疫刺激 乳腺 FcRn 基因表达 |
The Expression Level of FcRn in the Mice Mammary Gland Under the Natural and ISCOM Immune State |
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| ZhangChunlin , Liu Guanglei WangJiaqi Bu Dengpan Zhao Shengguo Zhou Lingyun Zhao Guoqi State Key Laboratory of Animal Nutrition.The Expression Level of FcRn in the Mice Mammary Gland Under the Natural and ISCOM Immune State[J].Journal of Guangxi Agricultural and Biological Science,2010(1):51-56. |
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| Authors: | ZhangChunlin Liu Guanglei WangJiaqi Bu Dengpan Zhao Shengguo Zhou Lingyun Zhao Guoqi State Key Laboratory of Animal Nutrition |
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| Institution: | ZhangChunlin 1,2 Liu Guanglei 1 WangJiaqi 1 Bu Dengpan 1 Zhao Shengguo 1 Zhou Lingyun 1 Zhao Guoqi 2 1 State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing,100193,2 College of Animal Science , Technology of Yangzhou University,Yangzhou,225009 |
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| Abstract: | The expression level of neonatal Fc receptor (FcRn) in the mammary gland of mice under different natural stages and the immune state were determined. One hundred Kunming white mice were divided into four groups. They were treated differently on 4 d after parturition as follows:group 1,injecting isotonic Na chloride; group 2,injecting Lipase+isotonic Na chloride; group 3,injecting ISCOM,and group 4,injecting Lipase-ISCOM. The specific IgG titer in serum and milk was determined by indirect ELISA at 8 d and 12... |
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| Keywords: | Mice Immune stimulation Mammary gland Fcrn Gene expression |
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