复垦煤矸山不同利用类型土壤微生物群落分析

土壤微生物是表征土壤质量状况的重要指标,因此研究其群落特征对指导煤矸山植被恢复具有重要意义。以山西霍州曹村矿煤矸山为对象,分析了农田(WL)、果园(PO)和草地(GL)3种土地利用类型0~10 cm和10~20 cm土层土壤微生物群落结构。结果表明:(1)3种复垦样地土壤微生物总数、微生物总浓度和微生物群落浓度均低于普通农田(CK),表明该煤矸山复垦地土壤质量状况仍未达到与普通农田相当的水平。(2)从土壤微生物群落结构看,3种土地利用类型中革兰氏阳性菌、细菌和放线菌所占比例无显著差异,而革兰氏阴性菌和真菌占比有显著变化。(3)在3种样地中,WL和PO样地中的优势菌种均为细菌16∶0、真菌18∶1 w9c和革兰氏阳性菌i15∶0、a15∶0;而GL样地中的优势菌种为细菌16∶0、真菌18∶1 w9c和革兰氏阳性菌i15∶0。

Analysis on Soil Microbial Community in Different Land Use Types on Reclamation of Coal Waste Pile

Soil microbe is an important index for evaluating soil quality,and thus investigating the characteristics of soil microbial community is important for vegetation restoration in coal waste pile.This study analyzed soil microbial community structure in 0-10 cm and 10-20 cm soil layers of three land-use types (cropland,WL;orchard,PO;and grassland,GL) in coal waste pile of Cao Village located in Huozhou,Shanxi Province.Results showed that 1) Soil microbial amount and total concentration and microbial community concentration were all lower in three kinds of reclamation land than in normal cropland (CK),which implied soil quality in reclaimed land of the coal waste pile had not reached the similar level as in the ordinary farmland yet.2) There was no significant difference in soil microbial community structure,such as the proportion of gram positive bacteria,bacteria and actinomycetes,while the proportion of gram negative bacteria and fungi varied significantly.3) The dominate strain was bacteria 16 ∶ 0,fungi 18 ∶ 1 w9c and gram positive bacteria i15 ∶ 0,and a15 ∶ 0 in WL and PO,while bacteria 16 ∶ 0,fungi 18 ∶ 1 w9c and gram positive bacteria i15:0 dominated GL.