两个新型超氧化物歧化酶模拟物的制备及其对玉米幼苗抗盐害作用研究

合成了两个新型羧甲基壳聚糖-铜(Ⅱ)-苯并咪唑衍生物型超氧化物歧化酶(SOD)模拟化合物:cmcts-Cu(Ⅱ)-tbz(1)和cmcts-Cu(Ⅱ)-hpb(2)(cmcts=羧甲基壳聚糖t,bz=2-(4′-噻唑基)苯并咪唑,hpb=2-(2′-吡啶基)苯并咪唑)。应用红外光谱,紫外-可见光谱,原子吸收光谱等对模拟物进行了表征,并研究了模拟物在中度土壤盐害的情况下对玉米幼苗抗盐害作用的影响。结果表明:在中度盐害情况下,经这些SOD模拟物浸种、叶面喷洒处理,玉米生物量得到较大提高,茎叶长和鲜重分别增加了7.30%～79.17%,和17.86%～119.05%,生物体内SOD活性提高了43.59%～80.22%,表明这些SOD模拟物能有效抑制玉米幼苗所受的盐害胁迫作用。

The Synthesis of Two New SOD Mimics and Their Effects on the Salt Resistance of Maize Seedlings

Two new superoxide dismutase(SOD)mimics: cmcts-Cu(Ⅱ)-tbz(1)and cmcts-Cu(Ⅱ)-hpb(2)(cmcts=carboxymethyl chitosan,tbz=2-(4’-thiazolyl) benzimidazole,hpb=2-(2-pyridyl) benzimidazole) were synthesized and characterized by infrared spectrum,electronic absorption spectrum and atomic absorption spectrometry,and the effects of the SOD mimics on the salt stress of maize seedlings were studied by the way of soaking and spraying 0.08-0.4 mg L-1 SOD mimics.The results showed that the SOD mimics increased the biomass 7.30%-79.17%(leaf),17.86% ~ 119.05%(fresh)],and enhanced the SOD activities(43.59%-80.22%) of the maize seedlings,indicating that the SOD mimics could effectively protect the maize seedlings against the salt stress.