| 棉花黄萎病生防菌脂肽类抑菌物质分离和鉴定 |
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| 引用本文： | 罗佳,陈立华,赵爽,杨兴明,冉伟,沈其荣,胡江.棉花黄萎病生防菌脂肽类抑菌物质分离和鉴定[J].土壤学报,2012,49(3):612-619. |
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| 作者姓名： | 罗佳 陈立华 赵爽 杨兴明 冉伟 沈其荣 胡江 |
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| 作者单位： | 1. 南京农业大学,江苏省固体有机废弃物资源化高技术研究重点实验室,南京210095;江苏省农业科学院农业资源与环境研究所,南京210014
2. 南京农业大学,江苏省固体有机废弃物资源化高技术研究重点实验室,南京210095 |
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| 基金项目： | 科技部重大成果转化项目（2009120）和江苏省重大科技成果转化项目（BA2008027） |
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| 摘 要： | 棉花黄萎病(Cotton Verticillium Wilt)是一种由大丽轮枝菌(Verticillium dahliae Kleb)引起的严重危害棉花生产的土传维管束病害1]。因大丽轮枝菌菌丝体产生的大量微菌核在土壤中存活时间长以及病原菌变异快等特点,造成培育抗病品种、使用化学药剂和利用新的栽培技术等方法防病效果均不理想。近年来,以生物防治为主的综合防治措施日益受到人们重视,并取得了一定效果2-4];该措施
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| 关 键 词： | 棉花黄萎病 微生物有机肥 枯草芽孢杆菌 脂肽类抑菌物质 丰原素 |
| 收稿时间： | 2011/4/26 0:00:00 |
| 修稿时间： | 2011/10/18 0:00:00 |
Isolation and identification of bacterial lipopeptide, an antagonistic substance, from Bacillus subtilis for biocontrol of cotton verticillium wilt |
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| Luo Ji,Chen Lihu,Zhao Shuang,Yang Xingming,Ran Wei,Shen Qirong and Hu Jiang.Isolation and identification of bacterial lipopeptide, an antagonistic substance, from Bacillus subtilis for biocontrol of cotton verticillium wilt[J].Acta Pedologica Sinica,2012,49(3):612-619. |
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| Authors: | Luo Ji Chen Lihu Zhao Shuang Yang Xingming Ran Wei Shen Qirong and Hu Jiang |
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| Institution: | Institute of Agricultural Resource and Environmental Sciences, Jiangsu Academy of Agricultural Sciences; Institute of Agricultural Resource and Environmental Sciences, Jiangsu Academy of Agricultural Sciences,,Jiangsu Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University,Jiangsu Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University,Jiangsu Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University,Jiangsu Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University,Jiangsu Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University,Jiangsu Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University |
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| Abstract: | Cotton Verticillium wilt has restricted cotton production. Biological control has become the important approach to control soil born disease. Bacillus subtilis HJ5 isolated from healthy cotton rhizosphere in a cotton field with severe verticillium wilt showed a strong antagonistic effect to Verticillium dahliae Kleb (V. dahliae). The inhibition rates of strain HJ5 was 76.2%, and fermentation broth also had inhibitory effects on Verticillium dahliae Kleb (V. dahliae). The disease reduction percentage was 80% in the field after application of the bioorganic fertilizer made from HJ5 and organic fertilizer. Lipopeptide antibiotic were isolated and identified from the strain HJ5. Three kinds of lipopeptide were obtained in the fermentation broth of strain HJ5, they were 1463Da and 1477Da Fengycin A and 1491Da Fengycin B. The strain HJ5 produced a variety of antibiotics to suppress V. dahliae growth, which is an important factor to control verticillium wilt by the strain HJ5. |
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| Keywords: | Cotton Verticillium wilt Bio-organic fertilizer Bacillus subtilis Lipopeptide antibiotic Fengycin |
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