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| 产漆酶真菌筛选及其对PAHs污染土壤修复的初步研究 | |
| 引用本文： | 潘澄,茆婷,吴宇澄,申卫收,钟文辉.产漆酶真菌筛选及其对PAHs污染土壤修复的初步研究[J].土壤学报,2011,48(6):1253-1259. |
| 作者姓名： | 潘澄 茆婷 吴宇澄 申卫收 钟文辉 |
| 作者单位： | 1. 南京师范大学环境科学与工程系,南京210097;中国科学院土壤环境与污染修复重点实验室(南京土壤研究所),南京210008 2. 南京师范大学环境科学与工程系,南京,210097 3. 中国科学院土壤环境与污染修复重点实验室(南京土壤研究所),南京,210008 |
| 基金项目： | 国际科学基金项目及南京师范大学“211工程”三期重点学科建设项目 |
| 摘 要： | 真菌漆酶可以高效转化多环芳烃(PAHs),因此,产漆酶真菌在PAHs污染土壤修复中极具应用前景。根据漆酶可将愈创木酚氧化为红色物质的特性,成功从土壤中筛选出一株能够分泌漆酶的真菌菌株F-1,初步鉴定该菌为疣孢漆斑菌(Myrothecium verrucaria)。通过Plackett-Burman试验对菌株F-1的产酶能力进行了分析,发现特定培养条件组合可将其酶活提高近300倍,达5628 U L-1,表明F-1的漆酶活性受到环境条件的显著影响。应用菌株F-1对PAHs污染土壤进行了初步修复研究,结果表明,接种F-1对菲、荧蒽、芘、苯并(a)蒽、屈、苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)芘、二苯并(a,h)蒽、苯并(g,h,i)苝、茚苯(1,2,3-cd)芘等11种PAHs均有不同程度的降解,提示产漆酶真菌在PAHs污染土壤修复中的应用潜力。 |
| 关 键 词： | 多环芳烃(PAHs) 土壤污染 真菌修复 漆酶 |
| 收稿时间： | 5/25/2010 5:13:03 PM |
| 修稿时间： | 2011/1/11 0:00:00 |
Screening for laccase-producing fungus and its potential in remediation of PAHs-contaminated soil |
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| PAN CHENG,MAO TING,WU YUCHENG,SHEN WEISHOU and ZHONG WENHUI.Screening for laccase-producing fungus and its potential in remediation of PAHs-contaminated soil[J].Acta Pedologica Sinica,2011,48(6):1253-1259. | |
| Authors: | PAN CHENG MAO TING WU YUCHENG SHEN WEISHOU and ZHONG WENHUI |
| Institution: | Pan Cheng1,2 Mao Ting1 Wu Yucheng2 Shen Weishou1 Zhong Wenhui1 (1Department of Environmental Science and Engineering,Nanjing Normal University,Nanjing210097,China)(2Key Laboratory of Soil Environment and Pollution Remediation,Institute of Soil Science,CAS,Nanjing 210008,China) |
| Abstract: | Fungal laccase can efficiently oxidize polycyclic aromatic hydrocarbons(PAHs),suggesting the potential of laccase-producing fungi for application to remediation of PAHs-contaminated soil.Based on its feature of being capable of oxidizing guaiacol into something red,a strain of laccase-producing fungus,F-1,was isolated from soil.According the BLAST alignment of near full-length 18S rRNA gene sequence,this strain is closely related to Myrothecium verrucaria.Both single factor and Plackett-Burman experiments w... |
| Keywords: | Polycyclic aromatic hydrocarbons (PAHs) soil pollution fungal remediation laccase |
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