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连作土壤微生物区系分析、调控及高通量研究方法
引用本文:薛超,黄启为,凌宁,高雪莲,曹云,赵青云,何欣,沈其荣.连作土壤微生物区系分析、调控及高通量研究方法[J].土壤学报,2011,48(3):612-618.
作者姓名:薛超  黄启为  凌宁  高雪莲  曹云  赵青云  何欣  沈其荣
作者单位:南京农业大学江苏省固体有机废弃物资源化利用高技术研究重点实验室,南京,210095
基金项目:国家自然科学基金项目(40871126),农业部农业公益性行业科研专项(201103004),江苏省科技成果转化专项资金项目(BA2008027)
摘    要:土壤连作后导致土壤微生物生态失衡、病原微生物富集、有益微生物减少,土壤微生物从细菌主导型向真菌主导型转化,使病原菌更容易侵染植物而引发植物的各种土传病害。施用微生物有机肥可以将失衡的微生物区系恢复到健康的状态,从而可以起到防治土传病害的作用。本文围绕连作引起的土壤中各大类微生物数量和种类的变化以及连作土壤施用微生物有机肥修复后其微生物数量和种类的变化进行了综述,并通过与传统技术比较,介绍了454高通量测序研究方法的优越性,指出了其在土壤微生物区系研究中的重要性。

关 键 词:微生物有机肥  调控  土壤微生物区系  连作  土传病害  454测序
收稿时间:7/12/2010 8:09:33 PM
修稿时间:3/8/2011 12:13:52 PM

Analysis, regulation and high-throughput sequencing of soil microflora in mono-cropping system
xuechao and shen qi rong.Analysis, regulation and high-throughput sequencing of soil microflora in mono-cropping system[J].Acta Pedologica Sinica,2011,48(3):612-618.
Authors:xuechao and shen qi rong
Institution:Nanjing Agriculture University,nanjing agriculture university
Abstract:Continuously cropping system of cash crops often destroyed soil microbial ecology with soilborn pathogens enriched but the number as well as species of beneficial strains decreased, leading to a shift from bacterial dominant type to fungi dominant one. Therefore, pathogens can more easily invade plant roots resulting in severe plant disease such as Fusarium wilt. Application of bio-organic fertilizers can reconstruct soil microbial community structure, consequently suppressing soilborne diseases. Advances on changes of microbial amount and diversity after continuously cropping and also the recovery tendency of soil microbial amount and diversity by applying bio-organic fertilizer were summarized. Furthermore, 454 pyrosequencing was introduced as a powerful tool by comparison with the traditional methods to analyze the microbial structure in the future.
Keywords:Bio-organic fertilizer  Regulation  Soil microflora  Continuously cropping  Soilborn disease  454 sequencing
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