一株具有多途径氮代谢功能的荧光假单胞菌

为研究全自养脱氮过程，采用斯凯尔曼亚硝化单胞杆菌培养基，从南京某城市污水处理厂序批式处理系统（SBR）中分离得到一株具有多途径氮代谢功能的荧光假单胞菌Pseudomonas fluorescens dN13。该菌株在有氧条件下，无有机碳源存在，能进行氧化NH3-N产能代谢生长；有有机碳源存在且NO2^- -N和NO3^- -N的浓度较高时，它能将NO2^- -N和NO3^- -N还原为NH3-N。在厌氧条件下，只有乙酸钠作碳源时，该菌株仍能进行硝酸亚硝酸还原（反硝化）作用。在这个具有多途径氮代谢功能的菌株细胞内，硝化反硝化过程耦合成为可能。

A STRAIN OF PSEUDOMONAS FLUORESCENS WITH MULTI-PATHS OF NITROGEN METABOLISM

To study on autotrophic removal of nitrogen from wastewater containing a high concentration of NH3-N,a strain of Pseudomonas fluorescens dN13 was isolated,by means of incubation in Skerman's medium for Nitrosomona,from the sequence batch reactor system(SBR)of a wastewater treatment plant in Nanjing.Under aerobic condition,this strain could oxidize NH3-N into NO-2-N and grow without organic carbon source;and reduce NO-2-N and NO-3-N into NH3-N with organic carbon source and adequate concentration of NO-2-N and NO-3-N,while under anaerobic condition,strain dN13 could still reduce NO-2-N and NO-3-N into NH3-N with sodium acetate as the sole carbon source.In the cells of strain dN13 with such multi-paths of nitrogen metabolism,coupling of nitrification and denitrification is feasible.