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美国8种土壤的活性腐植酸和难溶性钙质腐植酸盐中碳官能团的特性分析
作者姓名:HE Zhong-Qi  CAO Xiao-Yan  MAO Jing-Dong  T. OHNO  H. M. WALDRIP
作者单位:[1]USDA-ARS, Southern Regional Research Center, New Orleans LA 70124 (USA) [2]Department of Chemistry and Biochemistry, Old Dominion University, Norfolk VA 23529 (USA) [3]Department of Plant, Soil, and Environmental Sciences, University of Maine, Deering Hall, Orono ME 04469 (USA) [4]USDA-ARS, Conservation and Production Research Laboratory, Bushland TX 79012 (USA)
摘    要:Solid state13C nuclear magnetic resonance(NMR)spectroscopy is a common tool to study the structure of soil humic fractions;however,knowledge regarding carbon structural relationships in humic fractions is limited.In this study,mobile humic acid(MHA)and recalcitrant calcium humate(CaHA)fractions were extracted from eight soils collected from six US states and representing a variety of soils and ecoregions,characterized by this spectroscopic technique and analyzed for statistical significance at P≤0.05.We found that the abundances of COO and N–C=O functional groups in the MHA fractions were negatively correlated to soil sand content,but were positively correlated to silt,total N and soil organic carbon contents.In contrast,the abundances of the COO and N–C=O functional groups were only positively correlated to the content of clay in the CaHA fractions,indicating that the two humic fractions were associated with diferent soil components.The two13C NMR peaks representing alkyls and OCH3/NCH were negatively correlated to the peaks representing aromatics,aromatic C–O and N–C=O/COO.Comparison of the sets of data from13C NMR spectroscopy and ultrahigh resolution mass spectrometry revealed that the aromatic components identified by the two methods were highly consistent.The comparison further revealed that protein in MHA was associated with,or bound to,the nonpolar alkyl groups,but a component competitively against(or complementary to)aromatic groups in the MHA composition.These observations provided insight on the internal correlations of the functional groups of soil humic fractions.

关 键 词:官能团分析  有机碳含量  腐植酸钙  土壤  美国  提取  移动  腐殖质组分
收稿时间:15 November 2012

Analysis of carbon functional groups in mobile humic acid and recalcitrant calcium humate extracted from eight US soils
HE Zhong-Qi,CAO Xiao-Yan,MAO Jing-Dong,T. OHNO,H. M. WALDRIP.Analysis of carbon functional groups in mobile humic acid and recalcitrant calcium humate extracted from eight US soils[J].Pedosphere,2013,23(6):705-716.
Authors:HE Zhong-Qi  CAO Xiao-Yan  MAO Jing-Dong  T OHNO and H M WALDRIP
Institution:1USDA-ARS, Southern Regional Research Center, New Orleans LA 70124 (USA) 2Department of Chemistry and Biochemistry, Old Dominion University, Norfolk VA 23529 (USA) 3Department of Plant, Soil, and Environmental Sciences, University of Maine, Deering Hall, Orono ME 04469 (USA) 4USDA-ARS, Conservation and Production Research Laboratory, Bushland TX 79012 (USA)
Abstract:Solid state 13C nuclear magnetic resonance (NMR) spectroscopy is a common tool to study the structure of soil humic fractions; however, knowledge regarding carbon structural relationships in humic fractions is limited. In this study, mobile humic acid (MHA) and recalcitrant calcium humate (CaHA) fractions were extracted from eight soils collected from six US states and representing a variety of soils and ecoregions, characterized by this spectroscopic technique and analyzed for statistical significance at P = 0.05. We found that the abundances of COO and N-C=O functional groups in the MHA fractions were negatively correlated to soil sand content, but were positively correlated to silt, total N and soil organic carbon contents. In contrast, the abundances of the COO and N-C=O functional groups were only positively correlated to the content of clay in the CaHA fractions, indicating that the two humic fractions were associated with different soil components. The two 13C NMR peaks representing alkyls and OCH3/NCH were negatively correlated to the peaks representing aromatics, aromatic C-O and N-C=O/COO. Comparison of the sets of data from 13C NMR spectroscopy and ultrahigh resolution mass spectrometry revealed that the aromatic components identified by the two methods were highly consistent. The comparison further revealed that protein in MHA was associated with, or bound to, the nonpolar alkyl groups, but a component competitively against (or complementary to) aromatic groups in the MHA composition. These observations provided insight on the internal correlations of the functional groups of soil humic fractions.
Keywords:correlation analysis  humic substances  nuclear magnetic resonance  soil organic matter  ultrahigh resolution mass spectrometry
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