| Effect of environmental C/N ratio on activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium |
| |
| 作者姓名： | Saihua HUANG Deyin HUANG Qitang WU Meifang HOU Xiaoyan TANG Jian ZHOU |
| |
| 作者单位： | College of Natural Resources and Environment;School of Ecological Technology and Engineering;Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control |
| |
| 基金项目： | supported by the National Natural Science Foundation of China(Nos.41171250,20907011,and 41401356);the Natural Science Foundation of Guangdong Province,China(No.2014A030313702)。 |
| |
| 摘 要： | Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants(POPs).In this study,effect of environmental C/N ratio on the activities of lignin-degrading enzymes,lignin peroxide(Li P)and manganese peroxidase(Mn P),produced by Phanerochaete chrysosporium,a white rot fungus,was investigated.Glucose was used as C source,and ammonium tartrate of different concentrations was used as N source to provide different C/N ratios.Relationships between Li P and Mn P activities and environmental C/N ratio were explored.The results showed that the higher the N source concentration,the faster the mycelium pellets aged.The faster the mycelium dry weight increased,the higher the Li P and Mn P activities.A high C/N ratio was a necessary condition for the secretion of Li P or Mn P.In addition,mycelium dry weight essentially affected enzyme activities.In the 122 C/N ratio and 50 C/N ratio treatments,mycelium dry weight essentially affected Mn P activity and both Li P and Mn P activities,respectively.
|
| 关 键 词： | biodegration lignin PEROXIDE manganese PEROXIDASE PEROXIDASE persistent organic POLLUTANT polysaccharide white ROT FUNGUS |
| 收稿时间： | 13 October 2015 |
Effect of environmental C/N ratio on activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium |
| |
| Saihua HUANG,Deyin HUANG,Qitang WU,Meifang HOU,Xiaoyan TANG,Jian ZHOU.Effect of environmental C/N ratio on activities of lignin-degrading enzymes produced by Phanerochaete chrysosporium[J].Pedosphere,2020,30(2):285-292. |
| |
| Authors: | Saihua HUANG Deyin HUANG Qitang WU Meifang HOU Xiaoyan TANG and Jian ZHOU |
| |
| Institution: | 1College of Natural Resources and Environment, South China Agricultural University, 483 Wushan Street, Guangzhou 510642 (China);
2School of Ecological Technology and Engineering, Shanghai Institute of Technology, 100 Haiquan, Shanghai 201418 (China);
3Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-environmental and Soil Sciences, 808 Tianyuan Road, Guangzhou 510650 (China) |
| |
| Abstract: | Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants (POPs). In this study, effect of environmental C/N ratio on the activities of lignin-degrading enzymes, lignin peroxide (LiP) and manganese peroxidase (MnP), produced by Phanerochaete chrysosporium, a white rot fungus, was investigated. Glucose was used as C source, and ammonium tartrate of different concentrations was used as N source to provide different C/N ratios. Relationships between LiP and MnP activities and environmental C/N ratio were explored. The results showed that the higher the N source concentration, the faster the mycelium pellets aged. The faster the mycelium dry weight increased, the higher the LiP and MnP activities. A high C/N ratio was a necessary condition for the secretion of LiP or MnP. In addition, mycelium dry weight essentially affected enzyme activities. In the 122 C/N ratio and 50 C/N ratio treatments, mycelium dry weight essentially affected MnP activity and both LiP and MnP activities, respectively. |
| |
| Keywords: | biodegration lignin peroxide manganese peroxidase peroxidase persistent organic pollutant polysaccharide white rot fungus |
| 本文献已被 CNKI 维普 ScienceDirect 等数据库收录！ |
| 点击此处可从《土壤圈》浏览原始摘要信息 |
| 点击此处可从《土壤圈》下载免费的PDF全文 |
|
