Arsenic-contaminated soils genetically modified<Emphasis Type="Italic">Pseudomonas</Emphasis> spp. and their arsemc-phytoremediation potential |
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Authors: | Olga?I?Sizova Vladimir?V?Kochetkov Shamil?Z?Validov Alexander?M?Boronin Email author" target="_blank">Paul?V?KosterinEmail author Yelena?V?Lyubun |
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Institution: | (1) Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 5 Prospekt Nauki, Pushchino, 142290 Moscow Oblast, Russia;(2) Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410015 Saratov, Russia |
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Abstract: | Sorghum was inoculated withPseudomonas bacteria, including strains harboring an As-resistance plasmid, pBS3031, to enhance As-extraction by the plants.Pseudomonas strains (P.fluorescens 38a, P.putida 53a, and P.aureofaciens BS1393) were chosen because they are antagonistic to a wide range of phyto-pathogenic fungi and bacteria, and they can stimulate
plant growth. The resistance of natural rhizospheric pseudomonads to sodium arsenite was assessed. Genetically modifiedPseudomonas strains resistant to As(III)/As(V) were obtained via conjugation or transformation. The effects of the strains on the growth
of sorghum on sodium-arsenite-containing soils were assessed. The conclusions from this study are: (1) It is possible to increase
the survivability of sorghum growing in sodium-arsenite-containing soil by using rhizosphere pseudomonads. (2) The presence
of pBS3031 offers the strains a certain selective advantage in arsenite-contaminated soil. (3) The presence of pBS3031 impairs
plant growth, due to the As-resistance mechanism determined by this plasmid: the transformation of the less toxic arsenate
into the more toxic, plant-root-available arsenite by arsenate reductase and the active removal of arsenite from bacterial
cells. (4) Such a mechanism makes it possible to develop a bacteria-assisted phytoremediation technology for the cleanup of
As-contaminated soils and is the only possible way of removing the soil-sorbed arsenates from the environment. |
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Keywords: | Arsenic-contaminated soils As-resistance plasmid phytoremediation Pseudomonas rhizosphere |
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