Effects of tetracycline on the soil microflora: function,diversity, resistance |
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Authors: | Hund-Rinke Kerstin Simon Markus Lukow Thomas |
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Institution: | (1) Fraunhofer Institute for Molecular Biology and Applied Ecology, D-57377 Schmallenberg, Germany |
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Abstract: | Background Tetracycline is a widely used antibiotic in animal production. Significant amounts of the substance reach the soil via feces,
urine and manure application. As tetracycline is a persistent compound with antibacterial activity, its presence in soil may
have undesired direct and indirect effects. These have been investigated so far focusing on effects on selected microbial
functions.
Objectives The aim of the present study was to obtain comprehensive information on potential effects of tetracycline on the soil microflora
under environmentally relevant conditions. The investigations included function and structure of the microbial biocoenosis
and the distribution of resistance genes.
Methods Pig manure rich in tetracycline resistance genes was applied to a sandy soil. This soil as well as an unamended soil were
additionally treated with several concentrations of tetracycline. The spiked soils were incubated in outdoor lysimeters for
several months. Substrate induced respiration, PLFAs, ten selected resistance genes, and the concentrations of tetracycline
were determined.
Results The test concentrations, though far exceeding environmental relevance, caused only small effects. An establishment of resistance
could not be detected. Applied resistance genes were not detectable at the end of the study even in the presence of added
tetracycline.
Conclusion Due to the high sorption capacity of the antibiotic, environmentally relevant concentrations of tetracycline do not seem to
cause undesired effects on the soil microflora. |
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Keywords: | Antibiotics microbial function microbial structure soil tetracycline resistance |
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