草莓茎尖组织培养技术研究

为建立完善的草莓组培快繁体系，解决种苗退化问题，以当地草莓主栽品种红颜茎尖为试材，设置0.1%升汞和0.1%升汞+2%吐温-80溶液2种外植体消毒处理，以MS为基本培养基，分别添加不同浓度6-苄氨基腺嘌呤(6-BA)和萘乙酸(NAA)的6个诱导和继代增殖培养基、5个生根培养基，研究草莓茎尖外植体消毒最佳方式与时间， 筛选诱导、继代增殖及生根培养的最佳培养基。结果表明，0.1%升汞+2%吐温-80溶液处理8 min时草莓茎尖污染率及褐化率较低，萌发率最高，可达82.3%，为最佳的消毒方式。外植体在MS+1.0 mg/L 6-BA+0.2 mg/L NAA培养基中，可诱导出更多的健壮不定芽，萌发率为83.3%，为最佳诱导培养基；适宜的继代增殖培养基为 MS+1.0 mg/L 6-BA +0.02 mg/LNAA，增殖系数为4.5%；1/2MS+0.01 mg/L NAA为最佳生根培养基，生根率高达100％。

Study on the Tip-shoot Tissue Culture Techniques for Strawberry

In order to improve the strawberry tissue culture and rapid propagation system and to address the quality degradation issue in seedlings, taking tip-shoots of a main strawberry variety Hongyan as material, two explant disinfection methods were used i.e., 0.1% HgCl2 solution and 0.1% HgCl2 plus 2% Tween-80 solution, 6 induction media and multiplicative culture media and 5 rooting culture media based on MS culture base plus different concentrations of 6-BA and NAA were formulated to study the optimum explant disinfection method and time, and to select the optimum culture media for induction, multiplicative culture and rooting culture for tip-shoot tissue culture of strawberry, respectively. Results showed that 0.1% HgCl2 plus 2% Tween-80 solution treated for 8 min had the lowest pollution rate and browning rate of tip-shoot tissue, and the highest germination rate of 82.3%, which was considered as the optimum explant disinfection method. The optimum induction medium was MS plus 1.0 mg/L 6-BA plus 0.2 mg/L NAA which could induce more health adventitious buds with a germination rate of 83.3%. The optimum medium for multiplicative culture was MS plus 1 mg/L 6-BA plus 0.02 mg/L NAA which had a multiplication coefficient of 4.5%. The optimal medium for rooting was 1/2MS plus 0.01 mg/L NAA which showed a rooting rate of 100%.