利用SSR对60个玉米自交系进行杂种优势群划分初探

用SSR对60个玉米自交系的DNA进行分子标记和杂种优势群划分研究。利用14对SSR引物在供试材料中检测出57个等位基因变异,每对引物检测等位基因2～7个,平均为4．07个,多态信息量变化范围为0．389～0．832,平均为0．692。自交系间遗传相似系数变幅为0．058～0．756,UPGMA聚类分析表明,供试自交系可分为五个类群。利用这14对具有较高多态性信息量的引物,可以对供试材料进行初步鉴定。

Research of Partition of Heterosis Group among 60 Maize Inbred Lines with SSR

Simple sequence repeat(SSR)was used to detect genetic variation among 60 maize(Zea mays L.)inbred lines.14 SSR primers,57 alleles were identified among the inbred lines totally.The average number of alleles per SSR locus was 4.07 with a range of 2 to 7.The polymorphism information content(PIC)for the SSR loci varied from 0.389 to 0.832 with an average of 0.692.Genetic similarities among the 60 lines ranged from0.058 to 0.756.The cluster analysis showed that the inbred lines could be classified into five distinct clusters,which corresponded to the heterotie groups determined by their pedigree analysis. Fourteen SSR primers,which had high level of polymorphism,could allow A rapid and efficient identification of 60 inbred lines. Consequently,SSR markers could be used for measuring genetic variation and assigning maize inbred lines to heterotic groups.