| 百合农杆菌介导的遗传转化受体系统的建立 |
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| 引用本文： | 林敬晶,眭顺照,皮伟,秦华,李名扬.百合农杆菌介导的遗传转化受体系统的建立[J].南方农业,2007,1(6):7-10. |
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| 作者姓名： | 林敬晶 眭顺照 皮伟 秦华 李名扬 |
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| 作者单位： | 西南大学园艺园林学院，西南大学花卉研究所，西南大学园艺园林学院，西南大学花卉研究所，西南大学园艺园林学院，西南大学花卉研究所，西南大学园艺园林学院，西南大学花卉研究所，西南大学园艺园林学院，西南大学花卉研究所 重庆400715，重庆400715，重庆400715，重庆400715，重庆400715 |
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| 基金项目： | 重庆市自然基金项目(2005BB1129)，重庆市教委项目(KJ050209) |
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| 摘 要： | 以麝香百合"素雅"(white-Elegance)作为供试材料建立了百合农杆菌介导的受体系统。鳞茎片的诱导分化以MS培养基中添加0.5mg/L6-BA 0.2mg/LNAA为最好;再生苗鳞茎片的不定芽分化以MS培养基中添加1.0mg/L6-BA 0.2mg/LNAA为最好;再生苗小叶柄的诱导以MS培养基中添加1mg/LNAA为佳。确定了再生苗不同部位适宜的抗生素筛选浓度,卡那霉素的筛选浓度小鳞茎块确定为125mg/L,而小叶柄确定为75mg/L;头孢霉素抑菌浓度确定为250mg/L。
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| 关 键 词： | 百合 受体系统 |
Establishment of Acceptor System of Agrobacterium Mediated Transformation of Lily |
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| Lin Jingjing,Sui Shunzhao,Pi Wei,Qin Hua,Li Mingyang.Establishment of Acceptor System of Agrobacterium Mediated Transformation of Lily[J].South China Agriculture,2007,1(6):7-10. |
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| Authors: | Lin Jingjing Sui Shunzhao Pi Wei Qin Hua Li Mingyang |
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| Abstract: | With Lilium oriental hybrid"swhite-Elegance"as the study subject,a high-frequency regeneration system was constructed,The results showed that the medium suitable for inducing the scales to differentiate adventitious buds was MS 0.5 mg/L 6-BA 0.2 mg/L NAA , and the medium suitable for the petioles of the test- tube plantlets to differentiate adventitious buds was MS 1.0 mg/L 6-BA 0.2 mg/L NAA ,the medium suitable for the scale leaves of the test-tube plantlets to differentiate adventitious buds was MS 1.0 mg/L NAA. The concentrations of the antibiotics for different parts of the test-tube plantlets in their cultures were determined, the concentration of kanamycin being 125 mg/L for the petioles and 75 mg/L for the scales,and the concentra- tion of carbenicillin being 250 mg/L . |
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| Keywords: | lily acceptor system |
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