| 重金属超富集植物龙葵对镉响应的蛋白组学分析 |
| |
| 引用本文： | 罗 琼,葛 青,刘小京,谢志霞,张 苹,潘响亮,徐 进.重金属超富集植物龙葵对镉响应的蛋白组学分析[J].中国生态农业学报,2015,23(11):1429-1436. |
| |
| 作者姓名： | 罗 琼 葛 青 刘小京 谢志霞 张 苹 潘响亮 徐 进 |
| |
| 作者单位： | 青海师范大学生命与地理科学学院 西宁 810008,山西农业大学园艺学院 太谷 030801,中国科学院遗传与发育生物学研究所农业资源研究中心 石家庄 050022,中国科学院遗传与发育生物学研究所农业资源研究中心 石家庄 050022,青海师范大学生命与地理科学学院 西宁 810008,荒漠与绿洲生态国家重点实验室/中国科学院新疆生态与地理研究所 乌鲁木齐 830011,1. 青海师范大学生命与地理科学学院 西宁 810008 2. 山西农业大学园艺学院 太谷 030801 3. 中国科学院西双版纳热带植物园 勐腊 666303 5. 荒漠与绿洲生态国家重点实验室/中国科学院新疆生态与地理研究所 乌鲁木齐 830011 |
| |
| 基金项目： | 国家自然科学基金项目(31170228, 31272239)、河北省自然科学基金杰出青年基金项目(C2013503042)和荒漠与绿洲生态国家重点实验室开放基金项目资助 |
| |
| 摘 要： | 龙葵是典型的重金属超富集植物,但是我们对其重金属耐受和超富集的分子机理仍不完全清楚。为了从蛋白组学层面探究重金属超富集植物龙葵如何响应金属镉,本研究采用双向电泳和MALDI-TOF MS分析方法,鉴定了重金属超富集植物龙葵叶片和根中Cd胁迫下差异表达的蛋白。双向电泳在根和叶片中分别至少得到927和1 025个蛋白点,其中Cd胁迫下差异表达的蛋白点在根中有45个,叶片中有57个。采用MALDITOF MS分析,在根和叶片中分别鉴定了9个和12个蛋白点,分别代表了9个和6个差异表达的蛋白。生物信息学分析表明,这些蛋白涉及到激素合成、防御响应、能量代谢和细胞结构等。这些结果为进一步揭示重金属超富集植物龙葵响应Cd胁迫的分子调节机制,以及为通过现代生物技术手段进行重金属污染的植物修复提供了理论依据。
|
| 关 键 词： | 镉 龙葵 差异表达蛋白 双向电泳 质谱 生物信息学 |
| 收稿时间： | 3/1/2015 12:00:00 AM |
| 修稿时间： | 9/8/2015 12:00:00 AM |
Proteomic analysis of Cd-responsive proteins in hyper-accumulator Solanum nigrum |
| |
| LUO Qiong,GE Qing,LIU Xiaojing,XIE Zhixi,ZHANG Ping,PAN Xiangliang and XU Jin.Proteomic analysis of Cd-responsive proteins in hyper-accumulator Solanum nigrum[J].Chinese Journal of Eco-Agriculture,2015,23(11):1429-1436. |
| |
| Authors: | LUO Qiong GE Qing LIU Xiaojing XIE Zhixi ZHANG Ping PAN Xiangliang and XU Jin |
| |
| Institution: | College of Life Science and Geography, Qinghai Normal University, Xining 810008, China,College of Horticulture, Shanxi Agricultural University, Taigu 030801, China,Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China,Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China,College of Life Science and Geography, Qinghai Normal University, Xining 810008, China,State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China and 1. College of Life Science and Geography, Qinghai Normal University, Xining 810008, China 2. College of Horticulture, Shanxi Agricultural University, Taigu 030801, China 3. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China 5. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China |
| |
| Abstract: | Solanum nigrum is a typical heavy metal-hyper-accumulator. However, the molecular mechanisms underlying the tolerance and accumulation of heavy metal have remained largely unclear. To through light on the mechanism of Cd accumulation in Cd hyper-accumulator S. nigrum, we investigated the response of S. nigrum to Cd toxicity at root and leaf proteomic levels using 2-DGE technique. The root and leaf 2-DGE maps, respectively, consisted of at least 927 and 1 025 reproducible protein spots, of which 45 and 57 were classified as differentially expressed proteins. Through MALDI-TOF MS analysis, 9 and 12 of the spots identified, representing 9 and 6 proteins in root and leaf, respectively. The proteins were involved in phytohormone synthesis, defense responses, and energy metabolism and construction. This showed that proteomic analysis explained Cd response mechanisms in hyper-accumulator S. nigrum. It also provided theoretical basis for phytoremediation of heavy metal-contaminated soils through modern biotechnology. |
| |
| Keywords: | Cadmium Solanum nigurm Differentially expressed?protein 2-DGE technique Mass spectrum Bioinformatics |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国生态农业学报》浏览原始摘要信息 |
| 点击此处可从《中国生态农业学报》下载免费的PDF全文 |
|
