不同有机肥源对土壤微生物生物量及花生产量的影响

通过盆栽试验,采用平板计数法和DGGE分析法,研究施用化肥与不同来源的有机肥对土壤微生物生物量及花生产量的影响.结果表明,施肥均显著提高了花生的经济产量与生物产量,其中以施用麸酸有机复混肥处理最高;土壤中细菌、真菌、放线菌总量以施用鸡粪处理最高,其他处理差别不大;土壤微生物总DNA提取、PCR扩增及其产物DGGE分析表明,施用各品种有机肥较不施肥与施用化肥促进了土壤某些微生物量的提高,而施用不同有机肥品种促使不同种类微生物量的提高.故不同有机肥源对土壤微生物生物量乃至其多样性特征均产生影响.

Effects of different organic fertilizers on soil microbial biomass and yield of peanut

A pot experiment was set up to investigate the effects of different organic fertilizers on soil microbial biomass and yield of peanut. The results show that economic and biologic yields of peanut are improved by applying fertilizers. The highest yields are for the treatment with compound organic fertilizer containing monosodium glutamate. The total amount of bacteria, epiphyte and actinomyce of soil in the treatment with chicken faeces is highest among the different fertilizer treatments, while the other treatments have only minor differences. Through total DNA extraction, PCR amplification and DGGE analysis of soil microbe, it is found that the certain microbe population increases by the application of various organic fertilizers compared with the treatments of inorganic fertilizer and CK. Also the application of different organic fertilizers improves microbial biomass though to different degrees. Therefore different organic fertilizers affect both soil microbial biomass and diversity trait.