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黑豆种质苗期耐荫性评价及其根系对弱光胁迫的响应
引用本文:杨才琼,胡宝予,吴海军,秦雯婷,张潇文,刘卫国,杨文钰,刘江.黑豆种质苗期耐荫性评价及其根系对弱光胁迫的响应[J].中国生态农业学报,2017,25(6):893-902.
作者姓名:杨才琼  胡宝予  吴海军  秦雯婷  张潇文  刘卫国  杨文钰  刘江
作者单位:农业部西南作物生理生态与耕作重点实验室/四川农业大学生态农业研究所/四川省作物带状复合种植工程技术研究中心 成都 611130
基金项目:国家自然科学基金项目(31301277,31401329)和中国博士后科学基金面上项目(2014M560724)资助
摘    要:玉米-大豆复合种植是西南地区重要的生态种植模式之一,而目前耐荫型专用黑豆种质十分匮乏,黑豆的耐荫机理也尚不清楚,这严重影响了该模式的进一步推广。本研究旨在建立黑豆苗期耐荫性评价数学模型,筛选出能够判断黑豆耐荫性的有效指标,并探讨不同耐荫型黑豆根系对弱光胁迫的响应差异,为耐荫型黑豆种质的选育及耐荫机理的阐释奠定基础。试验以23个黑豆种质为研究材料,采用盆栽试验方法,设置自然光和遮荫2个处理。在黑豆植株V3期测定株高、茎粗、下胚轴长度、叶片干重、茎干重、主茎节数、总根长、根表面积、总根体积等形态指标和光合参数、叶绿素荧光参数等生理指标。采用多元统计方法建立黑豆苗期耐荫性评价模型,并根据评价结果,关联分析不同耐荫型黑豆根系对弱光胁迫的响应差异。通过逐步回归分析建立了黑豆苗期耐荫性评价数学模型;并通过该模型,筛选出蒸腾速率、株高、叶干重、最大荧光强度和初始荧光强度等5个鉴定指标。在荫蔽条件下测定苗期黑豆的上述5个指标可实现对黑豆苗期耐荫性的综合评价。同时,对23份黑豆种质的耐荫性综合评价值D进行了聚类分析,结果表明,黑豆种质可划分为耐荫型、中度耐荫型和荫蔽敏感型3类。不同耐荫型黑豆种质的根系结构对弱光胁迫的响应程度不同,黑豆的总根长、根表面积、总根体积、根干重等根系强度指标均表现为耐荫型中度耐荫型荫蔽敏感型。这表明耐荫潜力大的黑豆根系更发达。

关 键 词:黑豆  苗期  耐荫性  多元统计分析  根系结构
收稿时间:2016/12/2 0:00:00
修稿时间:2017/3/9 0:00:00

Evaluation for shade tolerance of black soybean germplasms and their root structure response to shade stress at seedling stage
YANG Caiqiong,HU Baoyu,WU Haijun,QIN Wenting,ZHANG Xiaowen,LIU Weiguo,YANG Wenyu and LIU Jiang.Evaluation for shade tolerance of black soybean germplasms and their root structure response to shade stress at seedling stage[J].Chinese Journal of Eco-Agriculture,2017,25(6):893-902.
Authors:YANG Caiqiong  HU Baoyu  WU Haijun  QIN Wenting  ZHANG Xiaowen  LIU Weiguo  YANG Wenyu and LIU Jiang
Institution:Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Institute of Ecological Agriculture, Sichuan Agricultural University/Sichuan Engineering Research Center for Strip Crop Intercropping System, Chengdu 611130, China,Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Institute of Ecological Agriculture, Sichuan Agricultural University/Sichuan Engineering Research Center for Strip Crop Intercropping System, Chengdu 611130, China,Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Institute of Ecological Agriculture, Sichuan Agricultural University/Sichuan Engineering Research Center for Strip Crop Intercropping System, Chengdu 611130, China,Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Institute of Ecological Agriculture, Sichuan Agricultural University/Sichuan Engineering Research Center for Strip Crop Intercropping System, Chengdu 611130, China,Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Institute of Ecological Agriculture, Sichuan Agricultural University/Sichuan Engineering Research Center for Strip Crop Intercropping System, Chengdu 611130, China,Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Institute of Ecological Agriculture, Sichuan Agricultural University/Sichuan Engineering Research Center for Strip Crop Intercropping System, Chengdu 611130, China,Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Institute of Ecological Agriculture, Sichuan Agricultural University/Sichuan Engineering Research Center for Strip Crop Intercropping System, Chengdu 611130, China and Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture/Institute of Ecological Agriculture, Sichuan Agricultural University/Sichuan Engineering Research Center for Strip Crop Intercropping System, Chengdu 611130, China
Abstract:Maize-soybean relay strip intercropping systems is an important planting mode in Southwest China. However, shade-tolerant germplasms of black soybean are scarce and the mechanism of shade tolerance of black soybean is still unclear, which significantly affects further promotion of the mode. The objective of this study was to establish a mathematical model to evaluate shade-tolerance ability of black soybean seedlings, screen shade-tolerance appraisal indicators and discuss the diverse responses of the root structures of different black soybean germplasms seedlings to shade stress. The study was also intended to lay the foundation for breeding varieties and for explaining the mechanism of shade tolerance of black soybean. Thus in the study, shade tolerance of 23 black soybean germplasms were evaluated in a pot culture experiment with normal light and shade treatments. The morphological characteristics (plant height, stem diameter, hypocotyl length, leaf dry weight, stem dry weight, main stem count, total root length, root surface area, total root volume, etc.) and physiological parameters were measured at the 3rd trifoliolate leaf stage (V3). An evaluation mathematical model was established using multivariate statistical analysis. Additionally, based on the evaluation results, correlation analysis was conducted to determine differential responses of roots to shade stress. The 23 black soybean germplasms were divided into three groups, including tolerant, moderately tolerant and sensitive germplasms. A total of 5 evaluation indexes closely related to shade tolerance were screened out. The study showed that the response of root structure to shade stress varied with shade-tolerance ability of black soybean germplasms. Root parameters such as total root length, root surface area, total root volume and root dry weight all had a similar order, which was shade tolerant germplasms > moderate shade tolerant germplasms > sensitive germplasms. The results indicated that shade tolerant germplasms of black soybean had a stronger root system.
Keywords:Black soybean  Seedling stage  Shade tolerance  Multivariable statistics  Root structure
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