黄绿木霉T1010对日光温室番茄耐冷生理指标的影响

采用黄绿木霉T1010(Trichoderma aureoviride1010)制剂处理山东寿光日光温室番茄连作土壤,通过人工控制日光温室内温度(白天16±2℃,夜间8±2℃),使供试番茄经过3d亚低温胁迫,分别测定其第3功能叶和根系的耐冷生理指标,探索黄绿木霉T1010对亚低温胁迫下番茄保护酶系统、非酶抗氧化物质类胡萝卜素、抗寒基因表达启动因素脱落酸(ABA),以及细胞内渗透调节保护物质脯氨酸和可溶性糖等耐冷生理指标的影响。结果表明:与常规生产区(对照)相比,黄绿木霉T1010处理番茄功能叶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)及多酚氧化酶(PPO)活力分别提高266.33%、16.85%、20.89%、632.89%,类胡萝卜素、脱落酸(ABA)、脯氨酸、葡萄糖、可溶性淀粉含量分别提高50.53%、119.40%、55.38%、128.57%和23.40%;番茄根系SOD、CAT、POD及PPO活力分别提高274.10%、69.06%、8.26%、164.14%,脯氨酸、葡萄糖、可溶性淀粉含量分别提高49.81%、66.00%和86.76%。1/2黄绿木霉T1010处理番茄功能叶以上耐冷生理指标除可溶性糖含量比对照有所降低外,其他指标均有不同程度的提高;番茄根系的相关耐冷生理指标则有不同程度的降低。ANOVA分析显示,亚低温胁迫下不同处理间日光温室番茄以上耐冷生理指标差异均达极显著水平。由此可见,在土壤有机质比较充裕的条件下,黄绿木霉T1010对亚低温胁迫下日光温室番茄功能叶和根系保护酶系统、类胡萝卜素、ABA、脯氨酸和可溶性糖等耐冷生理指标均有不同程度的积极影响。

Effect of Trichoderma aureoviride 1010 on chilly stress-resistance of tomato in solar-greenhouse conditions

In this study, we determined the enhancement effects of Trichoderma aureoviride 1010 (T1010) on chilly stress-resistance of tomato in solar-greenhouse conditions via soil application and 3 days of inferior chilly-stress (16 ± 2 °C in the day and 8 ± 2 °C at night ). Results show significant differences in the indicators for inferior chilly stress-resistance of functional tomato leaves and roots. SOD, CAT, POD and PPO activities of functional tomato leaves treated with T1010 increase by 266.33%, 16.85%, 20.89% and 632.89% respectively over the control. Carotenoid, ABA, proline, glucose and starch contents also increase by 50.53%, 119.40%, 55.38%, 128.57% and 23.40% respectively. For tomato roots treated with T1010, SOD, CAT, POD and PPO activities increase by 274.10%, 69.06%, 8.26% and 164.14% respectively. The contents of proline, glucose, and starch also increase by 49.81%, 66.00% and 86.76% respectively. In addition to decreasing soluble sugar content, the indicators for functional tomato leaves treated with 1/2T1010 increase at varying degrees. However, the indicators for tomato roots treated with 1/2T1010 decrease at varying degrees. ANOVA analysis shows significant difference in indicators of inferior chilly stress-resistance among CK, T1010 and 1/2T1010 treatments. T1010 positively influences chilly stress-resistance of functional tomato leaves and roots in solar-greenhouse conditions with reasonable soil organic quality.