菜籽多酚和Vc在化学模拟体系中清除超氧阴离子和羟自由基的能力

研究菜籽多酚、Vc、菜籽多酚与Vc混合物在化学模拟体系中清除超氧阴离子自由基OH.O2.与羟自由基.OH自由基的能力。用邻苯三酚自氧化法测定抗氧化剂清除OH.O2.的能力,用铁氧化邻二氮菲-Fe2+法测定抗氧化剂清除.OH的能力。结果表明:菜籽多酚、Vc、菜籽多酚与Vc的混合物清除OH.O2.、.OH都不呈剂量-效应关系。菜籽多酚在0.25mg/ml浓度时对OH.O2.与.OH的清除率均最高,分别达51.40%和84.82%。Vc在0.80mg/ml时对OH.O2.的清除率最高,达80.39%;在0.40mg/ml时对.OH的清除率最高,达91.35%。当总浓度为0.25mg/ml时,菜籽多酚与Vc的混合物没有协同增加清除OH.O2.的作用;菜籽多酚与Vc按7∶3、8∶2两种比例混合,在清除.OH时有协同增效作用,协同增效的最佳比例为7∶3,最大清除率达78.78%。

HYDROXYL RADICAL AND SUPEROXIDE RADICAL SCAVENGING ACTIVITIES OF POLYPHENOL FROM RAPESEED AND VC IN MODIFIED CHEMICAL SYSTEMS

The polyphenol from rapeseed(RSPP),vitamin C(Vc) and their mixture were analyzed for the antioxidant activity in different systems including hydroxyl radical(·OH) and superoxide anion radical(OH~·O~·_2) scavenge.The OH~·O~·_2 scavenging activities of RSPP and Vc were measured by pyrogallol auto-oxidation,and ·OH scavenging activity by phenanthrocine-Fe~(2+) system.The results showed that there(RSPP,VC and RSPP plus Vc) had no dose-effect relationships to activity of scavenging OH~·O~·_2 or ·OH.The highest OH~·O~·_2 or ·OH scavenging activity for RSPP measured at 0.25mg/ml.The highest scavenging rates for two radicds were 51.40% and 84.82%,respectively.The highest OH~·O~·_2 scavenging rate of V_c was 80.39% to at the concentration of 0.80mg/ml,and to ·OH at 0.40mg/ml.At the total concentration of 0.25mg/ml,the synergistic effect of RSPP plus Vc scavenging OH~·O~·_2 was not observed.For ·OH,the synergistic effects at the RSPP∶Vc ratio(7∶3 and 8∶2)were observed,and the highest scavenging rate was 78.78%(7∶3).