N+注入选育漆酶高产菌株及其产酶优化研究

以糙皮侧耳Pleurotus ostreatus WY01为出发菌株，通过N+注入诱变处理担孢子、RBBR-PDA平板变色法初筛、ATBS法测定漆酶活性复筛，获得1株漆酶高产诱变菌株ADW-08。用高碳低氮无机盐制备的油菜秸固体培养基（SM）培养，其峰值酶活力比出发菌株高出2.80倍，达7.78 U/g，且产酶稳定。对ADW-08固体培养产酶条件的研究表明，以葡萄糖为碳源明显优于蔗糖、麦芽糖、麸皮和可溶性淀粉；酒石酸铵较有利于ADW-08漆酶的分泌；适宜初始pH为5.0或6.0；ABTS和藜芦醇对产酶均有明显的诱导作用，而吐温-80对产酶有一定的抑制作用；正交试验结果表明，葡萄糖、酒石酸铵和pH最佳参数分别为15.0g/L、0.2g/L和5.2，酶活峰值为8.33 U/g。

Screening of Fungal Mutant Strain with High Laccase Yield by N+-Implantation and Enzyme Production Condition Optization

The basidiospores of Pleurotus ostreatus WY01 were exposed to N+ beam and the treated basidiospores were screened in RBBR-containing PDA plates.Then the laccase activities of the selected strains were determined by ABTS,and a mutant named ADW-08 with high yield of laccase was obtained.The maximum activity of laccase of ADW-08 increased up to 7.78 U/g,2.8 times of the original stain,and its laccase production ability was stable in high carbon and low nitrogen rape straw solid medium(SM).Results showed that g...