茶用菊苗期枯萎病抗性鉴定技术研究

为了建立茶用菊枯萎病抗性鉴定的方法,了解茶用菊种质资源对枯萎病抗性的差异,筛选抗性种质用于抗病育种,本研究以茶用菊为试验材料,通过枯萎病病原菌分离、形态学和真菌18S rDNA/ITS鉴定和致病力研究,开展茶用菊资源苗期枯萎病人工接种鉴定,并筛选优异抗病种质。结果表明,从发病的福白菊植株上分离到M15和M16 2株枯萎病病原菌菌株,经鉴定,这2株菌株均属于尖孢镰刀菌,致病力检测发现M15为强致病力菌株。通过枯萎病抗性鉴定,筛选到七月白1份高抗品种,杭白菊、苏菊7号等9份抗病品种,滁菊、亳菊等17份中抗品种,福白菊、苏菊6号等3份感病品种以及皇菊1份高感品种。本研究建立了一种高效的茶用菊品种抗枯萎病的鉴定方法,为茶用菊抗枯萎病品种改良奠定了基础。

Study on Identification Techniques of Resistance to Fusarium oxysporum at Seedling Stage of Chrysanthemum for Tea

In order to establish a method for identification of resistance to F. oxysporum, to understand the difference in resistance of tea germplasm resources to F. oxysporum, and screen resistant germplasm for disease resistance breeding. In this study, with pathogen isolate, morphology and fungal 18S rDNA/ITS identification and pathogenicity studies of F. oxysporum, inoculation identification of chrysanthemum for tea resources in seedling stage was screened out excellent disease-resistant germplasm. Two strains of F. oxysporum pathogens were isolated from the pathogenic fubaiju, which named M15 and M16. Pathogenicity test found that M15 is a stronger pathogenic strain. With identification of resistance to F. oxysporum, we screened out one high-resistance variety Qiyuebaibai, nine resistant varieties such as Hangbaiju, Suju NO.7, seventeen medium-resistant varieties such as Chuju and Boju, three susceptible varieties such as Fubaiju and Suju NO.6, and one high-sensitivity variety Huangju. This study established an efficient method for the identification of resistance to F. oxysporum in chrysanthemum for tea varieties, which laid the foundation for the improvement of resistance to F. oxysporum.