程序性翻译后修饰对FEN1功能调控的研究进展

瓣状核酸内切酶(FEN1)是结构特异性5′核酸酶超家族成员,以参与冈崎片段成熟、DNA重组、细胞凋亡DNA片段化和长片段碱基切除修复(LP-BER)而闻名,在生物体的多种代谢途径中发挥作用,对维持不同物种基因组的稳定性发挥着十分重要的作用。FEN1的功能或表达异常会导致生物体内的多个生命过程发生紊乱,如突变率增加、微卫星序列不稳定、DNA降解等,对生物体造成严重危害。因此,FEN1在生物体内的表达必须受到严格、精确、及时的调控。研究表明,翻译后修饰对FEN1蛋白的活性强弱、细胞定位及功能稳定性发挥着重要的调控作用。本文对关于FEN1翻译后修饰调控的相关研究进展进行总结,系统归纳翻译后修饰对FEN1功能的调控和影响,为后续开展FEN1程序性调控研究提供了参考。

Research Progress on Functional Regulation of Flap Endonuclease 1 by Programmed Post-Translational Modification

The flap endonuclease 1 (FEN1) is a member of the structure-specific 5' nuclease superfamily, known for participating in Okazaki fragment maturation, DNA recombination, apoptotic DNA fragmentation and long-fragment base excision repair (LP-BER). FEN1 plays its role in various metabolic pathways to maintain genome stability in different species. Abnormal expression of FEN1 in vivo can lead to disorders in many biological processes, such as increased mutation rate of autoimmune deficiency, unstable microsatellite sequence, DNA degradation and so on, resulting in serious damage to the organisms. Therefore, the expression of FEN1 in vivo must be regulated strictly, accurately and promptly. Recent studies have shown that post-translational modification of FEN1 plays an important role in regulating its activity, cellular localization and functional stability. In this paper, the research progress on the regulation of FEN1 post-translational modification was summarized, showing the regulation and influence of post-translational modification of FEN1 on its function, providing a basis for further study on the procedural regulation of FEN1.