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| Fe^＋离子注入诱变莲花突变体的SRAP分析 | |
| 引用本文： | 贾彦彦,邓传良,高俊,任映雪,王宁娜,高武军,张涛,李鹏飞,卢龙斗.Fe^＋离子注入诱变莲花突变体的SRAP分析[J].核农学报,2011,25(5):904-909,958. |
| 作者姓名： | 贾彦彦 邓传良 高俊 任映雪 王宁娜 高武军 张涛 李鹏飞 卢龙斗 |
| 作者单位： | 1. 河南师范大学生命科学学院,河南新乡,453007 2. 北京师范大学／北京市辐射中心,北京,100875 3. 北京莲花池公园,北京,100055 |
| 基金项目： | 国家自然科学基金项目(31000165); 河南省教育厅自然科学基金项目(2010B180016); 生命科学学院与北京辐射中心合作项目资助 |
| 摘 要： | 为了在分子水平上对离子注入诱变莲花突变体进行鉴定,本研究对同一剂量Fe^＋离子注入诱变的白洋淀红莲（Baiyangdian red lotus）突变体及其对照的基因组进行SRAP扩增,并利用非变性聚丙烯酰胺凝胶电泳对SRAP-PCR扩增产物进行分析。在优化好的SRAP体系基础上,从121对引物组合中筛选出了10对可以稳... |
| 关 键 词： | 白洋淀红莲 离子注入 SRAP |
| 收稿时间： | 2010-12-15 |
| 修稿时间： | 2011-03-06 |
SRAP ANALYSIS OF M_3 LOTUS MUTANTS INDUCED BY Fe~+ ION IMPLANTATION |
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| JIA Yan-yan DENG Chuan-liang GAO Jun REN Ying-xue WANG Ning-na GAO Wu-jun ZHANG Tao LI Peng-fei LU Long-dou.SRAP ANALYSIS OF M_3 LOTUS MUTANTS INDUCED BY Fe~+ ION IMPLANTATION[J].Acta Agriculturae Nucleatae Sinica,2011,25(5):904-909,958. | |
| Authors: | JIA Yan-yan DENG Chuan-liang GAO Jun REN Ying-xue WANG Ning-na GAO Wu-jun ZHANG Tao LI Peng-fei LU Long-dou |
| Institution: | JIA Yan-yan1 DENG Chuan-liang1 GAO Jun1 REN Ying-xue1 WANG Ning-na1 GAO Wu-jun1 ZHANG Tao2 LI Peng-fei3 LU Long-dou1 (1. College of Life Science,Henan Normal University,Xinxiang,Henan 453007,2. Beijing Normal University/Beijing Radiation Center,Beijing 100875,3. Lotus Pond Park of Beijing City,Beijing 100055) |
| Abstract: | To examine and determine the lotus mutants induced by the same Fe+ ion implantation at the molecular level,the SRAP technique was used,and the non denatured polyacrylamide gel electrophoresis was undertaken to analyze the PCR products. At the optimized SRAP reaction condition,of the 121 primer pairs tested,10 primer pairs could amplify stable and remarkable specific bands,with primer polymorphism of 8.26%. This 10 primer pairs amplified a total of 215 bands,83 of which were polymorphic,and the percent of po... |
| Keywords: | Baiyangdian red lotus ion implantation SRAP |
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