| 辐照对农产品中细菌ATP生物发光检测的干扰 |
| |
| 引用本文： | 高岳,冯敏,王泽港,吕海燕,马飞,史宙亮,葛才林,罗时石.辐照对农产品中细菌ATP生物发光检测的干扰[J].核农学报,2007,21(1):48-51. |
| |
| 作者姓名： | 高岳 冯敏 王泽港 吕海燕 马飞 史宙亮 葛才林 罗时石 |
| |
| 作者单位： | 1. 扬州大学江苏省遗传生理重点实验室,江苏,扬州,225009
2. 江苏省农业科学院原子能所,江苏,南京,200014 |
| |
| 摘 要： | ATP生物发光技术已广泛应用于样品含菌量的快速检测,但在应用该方法检测农产品的辐照灭菌效果时却出现了发光强度均高于对照的现象。本文分别从γ射线对ATP的影响、发光动力学以及发光强度增高的途径等方面对上述现象进行了研究。结果表明,发光强度的增加并非由ATP所造成引起的,而是由农产品中含有的细菌经辐照后引起的;干扰荧光素—荧光素酶制品中存在的能引起光辐射的物质是形成干扰的另一方面的原因。
|
| 关 键 词： | 辐照 灭菌效果检测 ATP生物发光 干扰 |
| 文章编号： | 1000-8551(2007)01-048-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 03 21 2006 12:00AM |
A PRELIMINARY STUDY OF IRRADIATION DISTURBANCE ON THE RAPID BACTERIA ASSAY WITH ATP BIOLUMINESCENCE TECHNIQUE FOR AGRICULTURAL PRODUCTS |
| |
| GAO Yue,FENG Min,WANG Ze-gang,LU Hai-yan,MA Fei,SHI Zhou-liang,GE Cai-lin,LUO Shi-shi.A PRELIMINARY STUDY OF IRRADIATION DISTURBANCE ON THE RAPID BACTERIA ASSAY WITH ATP BIOLUMINESCENCE TECHNIQUE FOR AGRICULTURAL PRODUCTS[J].Acta Agriculturae Nucleatae Sinica,2007,21(1):48-51. |
| |
| Authors: | GAO Yue FENG Min WANG Ze-gang LU Hai-yan MA Fei SHI Zhou-liang GE Cai-lin LUO Shi-shi |
| |
| Institution: | 1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou, Jiangsu 225009; 2. Institute of Atomic Energy, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu 210014 |
| |
| Abstract: | ATP bioluminescence technique has been widely used as a rapid method for detection of bacteria in sample,however,such a phenomenon that ATP bioluminescence value of irradiated sample was higher than that of control was observed.In order to make the phenomenon clear,effect of γ-ray irradiation on ATP, kinetics of luminescence and possible luminescence intensity increasing pathway were investigated.The results show that the increase of luminescence in irradiated food was not caused by ATP,is caused by the influence of the irradiated bacteria in sample; and probably by some materials that cause the light irradiation in luciferin-luciferase compound. |
| |
| Keywords: | irradiation sterilization determination ATP bioluminescence disturbance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
|
