| 一株极端耐辐射奇异球菌的分离和初步鉴定 |
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| 引用本文： | 陈明,刘秀敏,张维,林敏.一株极端耐辐射奇异球菌的分离和初步鉴定[J].核农学报,2007,21(1):44-47. |
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| 作者姓名： | 陈明 刘秀敏 张维 林敏 |
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| 作者单位： | 中国农业科学院生物技术研究所,北京,100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 从放射性污染的土壤样品中分离出1株具有极端耐辐射的微生物BR501,该菌最适生长温度为30℃,不具有氨苄青霉素、氯霉素、四环素、卡那霉素和利福平等抗性。UV、γ辐射生长曲线的结果显示BR501菌株具有极强的辐射抗性。BR501菌株的16S rDNA序列与多株Deinococcus属菌株有很高的同源性,其中,与D.radiodurans菌株的16S rDNA相似性高达99%。结合BR501菌株的表型和Deinococcus属具有代表性的D.RadioduransR1菌株的特征,将该菌株归属为Deinococcus属,初步命名为Deinococcus sp.BR501。
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| 关 键 词： | 耐辐射微生物 Deinococcus sp 16SrDNA |
| 文章编号： | 1000-8551(2007)01-044-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 06 5 2006 12:00AM |
SEPARATION AND CHARACTERIZATION OF A RADIORESISTANT BACTERIUM STRAIN BR501 FROM RADIATION POLLUTED SOIL |
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| CHEN Ming,LIU Xiu-min,ZHANG Wei,LIN Min.SEPARATION AND CHARACTERIZATION OF A RADIORESISTANT BACTERIUM STRAIN BR501 FROM RADIATION POLLUTED SOIL[J].Acta Agriculturae Nucleatae Sinica,2007,21(1):44-47. |
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| Authors: | CHEN Ming LIU Xiu-min ZHANG Wei LIN Min |
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| Institution: | Biotechnology Research Institute, Chinese Academy of Agricultural Sciences , Beijing 100081 |
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| Abstract: | Strain BR501,an extremely radioresistant bacterium isolated from the radioactive experimental soil.The optimal temperature for the growth of strain BR501 was 30℃.The UV radiation and γ-radiation survival curves showed the strain BR501 had highly radio-resistance.The strain was sensitive to Amp,Km,Rif,Cm and Tc.The 16S rDNA of the BR501 shared highly similarity to those of species in genus Deinococcus,especially to that of D.radiodurans R1(99%). Based on the 16S rDNA sequence analysis and the phenotype characteristics,the BR501 belongs to the evolution branch of Deinococcus and was designated Deinococcus sp.BR501. |
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| Keywords: | radioresistant bacterium Deinococcus sp 16S rDNA |
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