牡丹组织培养研究进展

本文从牡丹组织培养对品种、外植体的选择、基本培养基类型、生长调节物质选择和组培苗的移栽等方面论述了牡丹组织培养的研究现状。牡丹的组织培养中,外植体多采用胚、腋芽、顶芽、茎尖、幼叶、叶柄和土芽(地下芽)等,并且培养经过低温诱导后的鳞芽,可显著提高萌发率;在愈伤组织诱导试验中,选择分化程度较低的材料(如土芽),容易脱分化,获得愈伤组织;在牡丹的增殖培养中,6-BA起到了不可或缺的作用;生根培养中常使用的生长调节物质有IBA、NAA和IAA。此外,还探讨了牡丹组织培养研究中存在的问题,以及未来的研究和发展方向。

TISSUE CULTURE ADVANCES OF TREE PEONY

This paper summarized the advances of Paeonia suffruticosa tissue culture,including the varieties,explant selection,plant growth regulators,types of basic medium,transplantation and so on.Embryo,axillary bud,apical bud, stem apex,young leaf,petiole and underground bud are often adopted in Paeonia suffruticosa tissue culture,and germination rate of culturing scaly buds is obviously improved after low temperature induction.In the experiment of callus induction,the explants of low degree dedifferentiation(such as underground bud) is easier to get callus;6-BA is not absence in the proliferation culture;IBA,NAA and IAA are often used in the rooting culture of tree peony.Main problems in Paeonia suffruticosa tissue culture were analyzed,the research and development direction in the future was also discussed.