离子注入甜菊的诱变效应研究

用７５ｋｅＶ的氮和碳离子的不同剂量注入甜菊干种子，以γ射线作对比，研究其Ｍ１生物学效应和Ｍ２突变。结果表明，离子术能诱发甜菊染色体结构的变异，染色体畸变细胞率随离子注入剂量的提高呈增加趋势。离子束对甜菊的Ｍ１损伤效应低于γ射线，而诱发的Ｍ２突变高于γ射线。碳离子诱发的染色体畸变细胞率和Ｍ２有益性状突变高于氮离子。离子注入杂交一代丰１×日原和日原×丰２的诱变效应大于济宁种和丰２。

MUTAGENIC EFFECTS OF ION IMPLANTATION ON STEVIA

Dry seeds of Stevia were implanted by 75 keV nitrogen and carbon ions with various doses.The biological effects in M 1 and mutation in M 2 were studied.The results showed that ion beam was able to induce variation on chromosome structure in root tip cells.The rate of cells with chromosome aberration was increased with ion beam dose.The rate of cells with chromosomal aberration was lower than that induced with γ rays.Frequency of the mutation induced by implatation of N + and C + ions were higher than those induced by γ rays.The rate of cell with chromosome aberration and in M 2 useful mutation induced by implantation of C + ion was higher than those induced by implantation of N + ion.Mutagenic effects Feng 1 × Riyuan and Riyuan × Feng 2 by implantation of N + and C + were higher than that of Jining and Feng 2.