| 水稻空间诱变的遗传变异及突变体的AFLP分子标记 |
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| 引用本文： | 蒲志刚,张志勇,郑家奎,向跃武,张志雄,蔡平钟,文春描.水稻空间诱变的遗传变异及突变体的AFLP分子标记[J].核农学报,2006,20(6):486-489. |
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| 作者姓名： | 蒲志刚 张志勇 郑家奎 向跃武 张志雄 蔡平钟 文春描 |
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| 作者单位： | 1. 四川省农业科学院生物技术核技术研究所,四川,成都,610066
2. 四川省农业科学院水稻高粱研究所,四川,泸州,646000 |
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| 基金项目： | 国家高技术研究发展计划(863计划);四川省财政厅高新技术研究专项;四川省应用基础研究计划;四川省农业科学院青年基金 |
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| 摘 要： | 搭载“神舟三号”航天飞船的水稻纯合种子在返回地面后SP3代种子出现颖壳、茎杆和叶片的主脉变异为金黄色的突变体,其他性状无明显变异,经遗传分析,颜色变异属一对隐性基因控制的性状变异。同时利用AFLP分子标记的方法,对航天诱变的水稻突变体进行基因组对比分析,通过聚丙烯酰胺电泳寻找突变体(黄金1号)多态性DNA片段,经过一系列引物筛选,找到了5条多态性DNA条带,对开展突变体基因功能研究和杂交水稻聚合育种有十分重要的意义。
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| 关 键 词： | 空间诱变 突变体 颜色 AFLP 分子标记 |
| 文章编号： | 1000-8551(2006)06-486-04 |
| 收稿时间： | 12 28 2005 12:00AM |
| 修稿时间： | 2005年12月28 |
GENETIC VARIATION OF SPACE FLIGHT CARRIED RICE AND MUTANT ANALYSIS BY AFLP MOLECULAR MARKER |
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| PU Zhi-gang,ZHANG Zhi-yong,ZHENG Jia-kui,XIANG Yue-wu,ZHANG Zhi-xiong,CAI Ping-zhong,WEN Chun-miao.GENETIC VARIATION OF SPACE FLIGHT CARRIED RICE AND MUTANT ANALYSIS BY AFLP MOLECULAR MARKER[J].Acta Agriculturae Nucleatae Sinica,2006,20(6):486-489. |
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| Authors: | PU Zhi-gang ZHANG Zhi-yong ZHENG Jia-kui XIANG Yue-wu ZHANG Zhi-xiong CAI Ping-zhong WEN Chun-miao |
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| Institution: | 1. Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan; 2. Rice and Sorghum Institute, Sichuan Academy of Agricultural Sciences, Luzhou , Sichuan 646000 |
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| Abstract: | Rice seeds were carried by "Shenzhou No.3" space shuttle,a mutant with golden chaff,stem and leaf was selected and named Golden 1 after the seeds returned to the earth.Except the golden color,other traits of Golden 1 are no obviously different with its original material H9808.Genetic analysis identified that color variation was control by a pair of recessive gene.The DNA fragments of the mutant were compared with its parent by AFLP molecular markers.Five specific bands were found through a serial selections. |
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| Keywords: | AFLP |
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