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烟草钾转运体基因NtHAK1的克隆及表达模式分析
引用本文:鲁黎明,杨铁钊.烟草钾转运体基因NtHAK1的克隆及表达模式分析[J].核农学报,2011,25(3):469-476.
作者姓名:鲁黎明  杨铁钊
作者单位:1. 四川农业大学农学院,四川雅安625014;中国农业大学植物生理学与生物化学国家重点实验室,北京100193
2. 河南农业大学烟草学院,河南郑州,450002
基金项目:四川省烟草专卖局科技项目
摘    要:采用RT-PCR的方法,结合3'RACE与5'RACE,从普通烟草(Nicotiana tabacum)品种K326中克隆出烟草K+转运体基因NtHAK1的全长cDNA序列.该序列长度为2016bp,编码378个氨基酸,其分子量为42.27kDa,等电点7.2.生物信息学分析表明,该蛋白具有6个跨膜区,含有K+转运蛋白...

关 键 词:烟草  钾吸收转运基因  克隆  表达模式  生物信息学
收稿时间:2010-08-26
修稿时间:2010-12-14

CLONING AND EXPRESSION PROFILE ANALYSIS OF A PUTATIVE POTASSIUM TRANSPORTER GENE NTHAK1 IN TOBACCO
LU Li-ming,YANG Tie-zhao.CLONING AND EXPRESSION PROFILE ANALYSIS OF A PUTATIVE POTASSIUM TRANSPORTER GENE NTHAK1 IN TOBACCO[J].Acta Agriculturae Nucleatae Sinica,2011,25(3):469-476.
Authors:LU Li-ming  YANG Tie-zhao
Institution:1. Agronomy Department, Sichuan Agricultural University, Ya’an, Sichuan 625014; 2. State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100193; 3. Tobacco Department, Henan Agricultural University, Zhengzhou, Henan 450002
Abstract:Primers were designed by the homologous comparison of plant’s high-affinity potassium transporter genes. Full-length cDNA sequence of NtHAK1, a tobacco potassium transporter gene, was cloned using RT-PCR method combining 3’RACE and 5’RACE in Nicotiana tabacum cultivar K326.The sequence length of NtHAK1 is 2016bp, encoding a 378 amino acid protein, whose molecular mass is 42.27kDa and isoelectric point is 7.2.Protein structure and function analysis shows that the protein has six transmembrane domains and contains a functional K+ transporter protein conserved domains cl01227.Homology analysis shows that NtHAK1 shares high homology with arabidopsis, rice, barley and other plant’s high-affinity potassium transporters. At the plant organ level, NtHAK1 was expressed in roots, stems, leaves and flowers. Meanwhile, the expression of NtHAK1 was not induced by the external low potassium stress.
Keywords:tobacco  potassium transporter  cloning  expression profile  bioinformatics
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