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| 垂吊型矮牵牛叶片培养不定芽发生和微繁研究 | |
| 引用本文： | 臧运祥,张松,郑伟尉,陈美霞,达克东,吴禄平.垂吊型矮牵牛叶片培养不定芽发生和微繁研究[J].核农学报,2004,18(1):18-21. |
| 作者姓名： | 臧运祥 张松 郑伟尉 陈美霞 达克东 吴禄平 |
| 作者单位： | 1. 山东农业大学园艺学院,山东,泰安,271018 2. 山东潍坊职业技术学院,山东,潍坊,250086 3. 山东农业大学园艺学院,山东,泰安,271018;沈阳农业大学园艺学院,辽宁,沈阳,110161 4. 沈阳农业大学园艺学院,辽宁,沈阳,110161 |
| 摘 要： | 以垂吊型矮牵牛叶片为材料 ,建立了垂吊型矮牵牛叶片高效不定芽发生、植株再生和微繁的技术体系。研究了不同激素及其组合对叶片不定芽发生、壮苗和生根的效应。结果显示 ,MS +BA 2mg L +NAA 0 1mg L是叶片不定芽发生的适宜培养基 ;MS +BA 0 5mg L +IBA0 1mg L +GA 0 5mg L是不定芽形成壮苗的适宜培养基 ;MS +IAA 0 1mg L是壮苗生根的适宜培养基 ;草炭珍珠岩基质是生根苗移栽的适宜基质 |
| 关 键 词： | 垂吊型矮牵牛 叶片培养 不定芽 微繁殖 |
| 文章编号： | 1000-8551(2004)01-018-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2002年9月16日 |
ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATIONFROM LEAVES OF TRAILING PETUNIA(Petunia hybrida) |
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| ZANG Yun-xiang ZHANG Song Zheng Wei-wei CHEN Mei-xia DA Ke-dong , WU Lu-ping.ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATIONFROM LEAVES OF TRAILING PETUNIA(Petunia hybrida)[J].Acta Agriculturae Nucleatae Sinica,2004,18(1):18-21. | |
| Authors: | ZANG Yun-xiang ZHANG Song Zheng Wei-wei CHEN Mei-xia DA Ke-dong WU Lu-ping |
| Institution: | ZANG Yun-xiang 1 ZHANG Song 1 Zheng Wei-wei 1 CHEN Mei-xia 2 DA Ke-dong 1,3* WU Lu-ping 3 |
| Abstract: | High frequent adventitious shoot formation, plant regeneration and micro-propagation were established in trailing petunia(Petunia hybrida) leaf. Effect of different kind of hormone and its combination on adventitious shoot formation, plant regeneration and root regeneration was studied. The result showed that MS BA 2mg/L NAA 0.1mg/L was suitable for adventitious shoot formation from the leaf, the shoots grew well in MS BA 0.5mg/L IBA 0.1mg/L GA 0.5mg/L medium, MS IAA 0.1mg/L was the best medium for root regeneration. Turfysoil combined with perlite (1:1) was suitable for the growth of transplanted plantlet. |
| Keywords: | trailing petunia(Petunia hybrida) leaf culture adventitious shoot micro-propagation |
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