EMS诱导小麦品种烟农15突变体的鉴定和EST-SSR分析

用EMS对小麦品种烟农15进行诱变处理,以构建突变体库、创造小麦新种质,为小麦功能基因研究和小麦遗传改良提供基础材料。经过M2代筛选和M3代鉴定,得到11个农艺性状发生明显变异的突变系,其中籽粒大小和株高2个性状的变异幅度最大。11个突变系均有复合性状突变出现,将其分为3类突变表型:8个大粒、高秆突变系;2个半矮秆突变系;1个高秆、多蘖突变系。用715个EST-SSR引物对受体烟农15和4个M3突变系进行了分析,共有14个引物对在受体和突变系间能扩增出差异条带。其中12个引物对扩增结果的差异表现为条带的有无;2个引物对表现为扩增出长度不同的差异条带。

IDENTIFICATION AND EST-SSR ANALYSIS OF MUTANTS FROM WHEAT VARIETY "YANNONG 15" INDUCED BY EMS

The wheat variety "Yannong 15" were treated by EMS(ethyl methane sulfonate) for the construction of the mutant genebank and the creation of new germplasm,which provided the foundational materials for the study of wheat functional gene and wheat genetic improvement.After the selection from M_2 generation and the identification of M_3 generation,eleven mutant lines with the most significant variation on the traits of grain size and plant height,were obtained.All of the mutant lines are composited trait mutants,and according to variation character,they could be divided into three types: 8 mutant lines with large grain size and long-culm,2 mutant lines with semidwarf,1 mutant line with long-culm and multi-tiller.A total of 715 EST-SSR primer pairs were used to analyze "Yannong15" and 4 M_3 mutant lines.Polymorphic fragments were detected from 14 primer pairs,of which 12 showed polymorphism that with or without amplified fragment and 2 with different fragment length.